Chlorinated Amorphous Silica Carrier for Low-Denaturation Protein Binding
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing protein immobilization carriers, such as those made of zirconia and silica-based materials, suffer from protein denaturation due to planar rigid surfaces and complex preparation processes, leading to inadequate protein binding abilities.
Innovation Solution
A protein immobilization carrier composed of an amorphous silica-based compound with specific Si-NMR and FT-IR characteristics, containing chlorine, which reduces silanol groups and enhances Si-Cl bonds, allowing for a simpler production method and improved protein binding without denaturation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If zirconia-based protein immobilization carrier is used, then protein can be adsorbed and immobilized on the carrier, but the protein will have a planar rigid surface and denature, resulting in insufficient protein binding ability
Solution Approach 1:
The patent changes the chemical composition parameters of the silica-based carrier by controlling the SiO2 content (70-90 wt%), Cl content (1-30 wt%), and Na content (1-30 wt%), transforming the carrier from a conventional inert material to one with controlled reactivity and surface properties that prevent protein denaturation while maintaining binding ability
Solution Approach 2:
The patent creates a composite material system combining silica-based compound with specific chlorine and sodium content, forming a new type of carrier that integrates the benefits of silica structure with modified surface chemistry to simultaneously achieve protein immobilization and prevent denaturation
2Reliability
If silica-based biomolecule carrier with linkers is used, then proteins can be conjugated to the carrier, but the preparation process becomes complicated and proteins are prone to denaturation due to highly reactive silanol groups
Solution Approach 1:
The patent extracts and eliminates the problematic linkers and bioactive moieties from the carrier structure, using instead a simplified silica-based carrier with controlled Cl and Na content that directly provides the necessary protein binding capability without requiring additional conjugation steps
Solution Approach 2:
The carrier itself provides the protein binding function through its controlled chemical composition (SiO2, Cl, Na ratios), eliminating the need for external linkers and bioactive moieties to mediate the protein immobilization process
3Adaptability or versatility
If silica-based carrier with large number of silanol groups is used, then the carrier is highly reactive for protein conjugation, but proteins adsorbed on linkers are prone to denaturation
Solution Approach 1:
The patent precisely controls the chemical composition parameters, particularly the Cl content (1-30 wt%) and Na content (1-30 wt%), to modulate the reactivity of silanol groups, reducing excessive reactivity that causes protein denaturation while maintaining sufficient reactivity for protein binding
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The carrier effectively prevents protein denaturation and enhances protein binding capabilities while maintaining a stable protein-protein immobilization complex, suitable for use in antibody test kits and other applications.
Implementation Method 1
a protein immobilization carrier including an amorphous silica-based compound including silicon (Si), oxygen (O), and chlorine (Cl)
Data Source
Figure 1A~1C
Figure 2
Figure 3A~3B
AI summary
Provided are: a protein immobilization carrier that can be produced with a simple method, exhibits little degeneration of protein that has been adsorbed, and demonstrates a high protein-binding activity; and a protein immobilization carrier dispersion liquid, a protein immobilization carrier hydrate, a protein support complex, and an antibody testing kit that use the protein immobilization carrier. The protein immobilization carrier is composed of an amorphous silica-based compound containing silicon (Si), oxygen (O), and chlorine (Cl). When, in a solid-state 29Si-NMR spectrum of the carrier, the peak area derived from Si(OSi)4 is noted as Q4, the peak area derived from HO-Si(OSi)3 is noted as Q3, and the peak area derived from (HO)2-Si(OSi)2 is noted as Q2, (Q2 + Q3) / (Q2 + Q3 + Q4) is 0.30 or less. The molar concentration of chlorine atoms in the carrier as measured by X-ray fluorescence (XRF) analysis is 0.01 mol% or more.