Chlorophyll Extract Stabilization Using Squalene-Rich Storage Oils

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Solution Overview

Problem

Chlorophyll is unstable in the surrounding environment, making it difficult to apply to the food industry due to degradation from heat, light, oxygen, enzymes, and pH changes, and existing stabilization methods provide only short-term stability.

Innovation Solution

A method involving the use of oils with long-chain unsaturated lipid structures, such as squalene, to stabilize chlorophyll by dissolving it in these oils and storing it under controlled conditions, including supercritical fluid extraction and fractional extraction.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If chlorophyll-containing extract is stored at low temperature to maintain stability, then the storage stability is improved, but the storage cost and energy consumption increase

Engineering Contradiction:
Improvestorage stabilityVSAvoidenergy consumption
Core Design Contradiction:
Stability of the object's compositionVSUse of energy by stationary object

Solution Approach 1:

The patent changes the chemical composition parameters of the storage medium by adding specific antioxidants (vitamin C, vitamin E, BHA, BHT) and adjusting sugar content (10-50% w/v), thereby improving chlorophyll stability without requiring low-temperature storage conditions

Inventive Principle:
Principle #35Parameter changes

2Use of energy by stationary object

If conventional storage methods are used to reduce storage cost, then the storage cost is reduced, but the storage stability deteriorates due to bacterial growth and pigment degradation

Engineering Contradiction:
Improvestorage costVSAvoidstorage stability
Core Design Contradiction:
Use of energy by stationary objectVSStability of the object's composition

Solution Approach 1:

The patent introduces intermediary substances (antioxidants and preservatives) into the storage medium that mediate between the chlorophyll and harmful environmental factors (oxygen, bacteria), protecting the chlorophyll without requiring expensive low-temperature storage infrastructure

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The storage medium is formulated as a composite system containing sugar solution, antioxidants (vitamin C, vitamin E, BHA, BHT), and preservatives (sodium benzoate, potassium sorbate), where each component contributes specific protective functions to achieve stable chlorophyll storage at ambient temperature

Inventive Principle:
Principle #40Composite materials

3Adaptability or versatility

If natural pigments like chlorophyll are used to provide natural color, then the naturalness is improved, but the color stability deteriorates under light and heat exposure

Engineering Contradiction:
ImprovenaturalnessVSAvoidcolor stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent converts harmful factors (light, heat, oxygen) into manageable conditions by using antioxidants that specifically target and neutralize the harmful effects of these factors, allowing natural chlorophyll to maintain its color stability under normal storage conditions

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The antioxidant system creates a chemically protective environment that acts as an inert barrier between the chlorophyll and reactive oxygen species generated by light and heat exposure, preventing oxidation and color degradation

Inventive Principle:
Principle #39Inert atmosphere (Inert environment)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method achieves long-term stability of chlorophyll, allowing it to remain stable for over 10 months, and enables direct application in food and medicine without additional processing, reducing operational costs and environmental impact.

Implementation Method 1

antioxidants such as vitamin C and vitamin E to maintain the color stability of the extract

Methodology Applied
Scientific EffectOxidation: Oxidation

Data Source

PatentEP3858152B1Method for long-term storage of chlorophyll-containing extract
Publication Date: 2026.04.29 MYUNG EUN LEE
  • EP3858152B1 patent drawingFigure 1
  • EP3858152B1 patent drawingFigure 2(A)~2(D)
  • EP3858152B1 patent drawingFigure 3~4

AI summary

Provided is a method for long-term storage of chlorophyll, which is unstable in the surrounding environment, wherein a stabilizer employing oils having long-chain unsaturated lipid structures, of which squalene is representative, is used to stabilize chlorophyll.