Chorion Colony-Forming Cells for High-Yield Stem Cell Isolation
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for obtaining stem cells from adult sources, such as bone marrow and placenta, face challenges including low yield, high cost, labor-intensity, and the risk of chromosomal aberrations and tumor formation due to the need for multiple cell passaging, while existing placental perfusion techniques are impractical due to rapid cell loss and limited cell collection from non-perfused regions.
Innovation Solution
Development of novel chorionic colony-forming unit cells (CCFUC) derived from human placental chorion, characterized by expression of ACBD6, low telomerase activity, and ability to differentiate into all three germ layers without forming teratomas, which can be obtained through mechanical processing and filtration without enzymatic digestion, allowing for high-yield isolation and therapeutic use.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If stem cells are obtained from adult sources through multiple cell passaging, then sufficient amount of stem cells can be obtained, but chromosomal aberrations and tumor formation risk increase
Solution Approach 1:
The patent uses enzymatic digestion (collagenase, dispase, trypsin) and mechanical dissociation to release stem cells from placental tissue in advance, creating a single-cell suspension that can be directly cultured without multiple passaging steps. This preliminary preparation allows direct expansion of stem cells while maintaining genomic stability.
Solution Approach 2:
The patent optimizes culture conditions including serum concentration (10-20% FBS), pH (7.2-7.4), temperature (37°C), and CO2 concentration (5%) to maintain stem cell proliferation and genomic stability. These parameter optimizations enable high-yield cell production without the need for excessive passaging.
2Quantity of substance
If placental perfusion techniques are used to obtain stem cells, then stem cells can be collected, but rapid cell loss and limited collection from non-perfused regions occur
Solution Approach 1:
The patent extracts stem cells directly from placental tissue using enzymatic digestion and mechanical dissociation, bypassing the need for perfusion through vascular channels. This approach accesses stem cells from all regions of the placenta including non-perfused areas, eliminating cell loss associated with perfusion techniques.
Solution Approach 2:
The patent uses enzymatic agents (collagenase, dispase, trypsin) as intermediaries to break down tissue matrix and release stem cells from placental structures. These enzymes act as mediators that facilitate cell release without requiring vascular perfusion, thereby preventing cell loss.
3Productivity
If mechanical processing and filtration without enzymatic digestion is used to obtain CCFUCs, then high-yield isolation is achieved, but cell separation complexity increases
Solution Approach 1:
The patent segments the placental tissue into smaller pieces through mechanical mincing before filtration. This segmentation increases the surface area for enzyme action and facilitates subsequent cell release, enabling high-yield isolation while managing processing complexity through staged treatment.
Solution Approach 2:
The patent combines mechanical processing (mincing, filtration) with enzymatic digestion in a sequential workflow. This merging of physical and chemical methods achieves high-yield cell isolation while distributing the complexity across multiple manageable steps rather than requiring a single complex procedure.
Data Source
AI summary
The present invention features colony-forming unit cells derived from the chorion of human placenta and describes compositions and methods for the uses of chorionic cells and their products for therapeutic purposes based upon production and release of multiple growth factors and cytokines by these cells stimulating tissue regeneration independent of engraftment, as well as differentiation into a specific cell type.