Chromatogram Peak Analysis Using Mobile Phase Arrival Timing
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Solution Overview
Problem
Existing chromatogram analysis apparatuses struggle to differentiate between noise peaks caused by rapid changes in mobile phase composition or sample injection and actual component peaks, leading to compromised analysis reliability and omission of intended analysis targets.
Innovation Solution
A chromatogram analysis apparatus that automatically determines an analysis range by calculating mobile phase and sample arrival times based on system volume, flow rate, and composition information, excluding noise peaks caused by composition and injection changes from the analysis target.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If noise peaks are manually removed before analysis, then analysis reliability is improved, but operation complexity increases
Solution Approach 1:
The analysis range determination unit performs preliminary action by automatically identifying and excluding noise peaks (composition-derived peaks and injection-derived peaks) before the peak analysis unit executes the main analysis. This preliminary exclusion of interference peaks based on timing information resolves the contradiction by maintaining high analysis reliability while eliminating the need for manual noise peak removal, thus reducing operation complexity.
2Measurement precision
If manual peak identification is performed, then measurement precision is improved, but time consumption increases
Solution Approach 1:
The system uses feedback from timing information (mobile phase arrival time and sample arrival time) to automatically determine the analysis range and identify valid peaks. The analysis range determination unit receives timing data and uses it to exclude noise peaks, providing feedback-based automatic peak identification that maintains measurement precision while eliminating manual intervention and reducing time consumption.
3Productivity
If analysis range is expanded to include all peaks, then completeness of analysis is improved, but noise peak inclusion increases
Solution Approach 1:
The system segments the chromatogram into different time regions based on mobile phase arrival time and sample arrival time. The analysis range determination unit divides the total analysis period into a pre-analysis period (before sample arrival) and an analysis period (after sample arrival), excluding the pre-analysis period from peak analysis. This segmentation maintains analysis completeness for all sample-derived peaks while automatically excluding composition-derived noise peaks that occur earlier.
Data Source
AI summary
A chromatogram analysis apparatus comprises a system information storage unit configured to store a system volume, which is a volume of piping through which a sample of a fluid chromatograph passes; an analysis information storage unit configured to store a chromatogram acquired by an analysis performed by the fluid chromatograph, flow rate information regarding a flow rate of a mobile phase during the analysis, and composition information regarding a composition of the mobile phase in each time period during the analysis; a time calculation unit configured to calculate a mobile phase arrival time, which is a time required for a mobile phase delivered from a liquid sending unit of the fluid chromatograph to reach a detector, and a sample arrival time, which is a time required for a sample injected into the mobile phase to reach the detector, based on the system information, the flow rate information, and the composition information; an analysis range determination unit configured to determine a target range for peak analysis on the chromatogram based on the mobile phase arrival time and the sample arrival time, such that a composition-derived peak, which is a peak caused by a rapid change in the composition of the mobile phase, and an injection-derived peak, which is a peak caused by the injection of the sample into the mobile phase, are excluded from the analysis target; and a peak analysis unit configured to execute the peak analysis within the analysis range determined by the analysis range determination unit.