Chromatogram Display Gain Control for Hemoglobin Analysis
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Solution Overview
Problem
In HPLC chromatography, the peak of hemoglobin A0 is often 100 times higher than hemoglobin A1c, making it difficult to display the entire peak of hemoglobin A1c, and the presence of variant hemoglobins can lead to negative measurement errors, requiring a method to prioritize and enhance the visibility of important peaks in the chromatogram.
Innovation Solution
A method that determines the display form of the chromatogram based on priority levels of measurement target components, calculating the peak area and component ratio to adjust the display gain, ensuring that the peak of the highest priority component is displayed prominently, allowing for better recognition and visualization of important peaks.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the display gain is determined based on the peak height of HbA1c, then the HbA1c peak can be displayed appropriately, but the entire peak of HbA1c cannot be displayed due to the much higher HbA0 peak
Solution Approach 1:
The patent applies different display gains to different regions of the chromatogram. Specifically, the HbA1c region uses a first display gain determined by HbA1c peak height, while the HbA0 region uses a second display gain determined by HbA0 peak height. This local differentiation allows each region to be displayed with appropriate scaling, solving the contradiction between accurate HbA1c measurement and overall chromatogram visibility.
2Loss of information
If the display gain is switched between HbA0 region and HbA1c region based on variant Hb presence, then variant Hb peaks can be displayed, but the visual balance of the chromatogram deteriorates
Solution Approach 1:
The patent maintains different display gains for different regions regardless of variant Hb presence. The HbA0 region consistently uses a display gain determined by HbA0 peak height, while the HbA1c region uses a display gain determined by HbA1c peak height. This consistent regional differentiation preserves both variant Hb detection capability and chromatogram visual balance.
Solution Approach 2:
The chromatogram is segmented into distinct regions (HbA0 region and HbA1c region) with independent display gain control. This segmentation allows each region to be optimized independently for its specific components, enabling simultaneous display of variant Hb peaks in the HbA0 region and HbA1c peak in the HbA1c region without compromising visual balance.
3Measurement precision
If the peak of hemoglobin S is displayed preferentially, then hemoglobin S can be recognized more easily, but the visual balance of the chromatogram may be affected
Solution Approach 1:
The patent applies a display gain to the HbA0 region that is determined by the peak height of HbA0, which automatically scales the display of all peaks in that region including hemoglobin S. This regional approach allows hemoglobin S to be displayed with appropriate prominence relative to HbA0 while maintaining overall visual balance, rather than applying preferential enhancement that would disrupt the chromatogram's aesthetic and interpretive balance.
Data Source
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AI summary
A method for displaying a chromatogram as a combination of a HbA1c region where HbA1c elutes and a HbA0 region where HbA0 and one or more variant Hb's elute, the method including the steps of: determining, based on priority levels of HbA0 and one or more variant Hb's, a display form of the HbA0 region so that a peak of a component with higher priority level among HbA0 and one or more variant Hb's whose peaks are to be displayed in the HbA0 region is displayed more preferentially; and displaying the HbA0 region in the determined display form and the HbA1c region, concurrently.