Chromatogram Miniatures for Liquid Chromatography Parameter Influence
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Solution Overview
Problem
Designing a liquid chromatography protocol for purifying target molecules is challenging due to the need to determine the influence of various experimental parameters, which requires evaluating numerous data points to optimize purity, yield, and cost while minimizing time in drug development processes.
Innovation Solution
A method and system that automatically organize and visualize the impact of different experimental conditions by displaying chromatogram-miniatures in a diagram, allowing users to quickly identify trends and make decisions on purification protocols.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If automated liquid chromatography systems are used to quickly test different experimental conditions, then productivity is improved, but the quantity of data generated increases making evaluation difficult and time-consuming
Solution Approach 1:
The patent creates visual copies (chromatogram miniatures) of the actual chromatography data that can be displayed in a condensed format. These miniatures represent complex chromatographic separations in a simplified visual form that shows key features like peak positions, heights, and areas, allowing rapid comparison across multiple experimental conditions without analyzing the full raw data sets.
Solution Approach 2:
The patent transforms the data representation by adding a visual dimension to the chromatography results. Instead of presenting data in traditional tabular or detailed graphical formats, the invention projects chromatogram information into a two-dimensional miniature visualization that preserves essential separation characteristics while condensing the information density, enabling simultaneous viewing of multiple experimental outcomes.
2Measurement precision
If detailed chromatogram analysis is performed to accurately determine the influence of experimental parameters, then measurement precision is improved, but the time required for evaluation increases
Solution Approach 1:
The patent extracts the essential information from complex chromatograms by identifying and displaying only the most relevant features in the miniature representations. Key parameters such as peak positions, relative heights, and areas are extracted and visualized in a condensed format that maintains analytical value while eliminating redundant detail, allowing rapid assessment of experimental parameter effects.
Solution Approach 2:
The patent segments the chromatogram information into distinct visual elements within the miniatures, separating key features like individual peaks and their characteristics. This segmentation allows the user to quickly scan and compare specific aspects of different chromatograms without being overwhelmed by the continuous, detailed data, thereby maintaining measurement precision while reducing evaluation time.
Data Source
AI summary
Method for determining the influence of least a first and a second experimental parameter on a liquid chromatography protocol for purifying one or more target molecules from a sample, comprising the steps: —performing chromatography purifications of the sample at a plurality of different experimental conditions where at least the first and the second experimental parameter each are varied over a predetermined range, each purification being registered as a chromatogram by monitoring an output parameter indicative of the purification result during the purification; and—displaying in a graphical user interface at least a subset of the registered chromatograms as chromatogram-miniatures in an evaluation diagram wherein the position of each displayed chromatogram-miniature is determined by the experimental parameters for the corresponding purification, thereby allowing a user to visually determine trends and the influence of the experimental parameters on the liquid chromatography protocol.