Preparative Chromatograph System with Dynamic Injection Control
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Solution Overview
Problem
In stack injection fractionation collecting, fluctuations in room temperature and pump pressure lead to changes in retention time, reducing the reproducibility of chromatograms and causing insufficient separation and decreased recovery rates of components.
Innovation Solution
A preparative chromatograph system that includes a controller and a fractionation collecting condition correction part, which detects changes in chromatograms during stack injection and corrects the fractionation collecting conditions to maintain reproducibility by adjusting parameters such as flow rate and injection interval.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If stack injection is used to improve throughput, then productivity increases, but retention time fluctuates due to temperature and pressure changes, causing chromatogram reproducibility to deteriorate
Solution Approach 1:
The system monitors retention time in real-time during stack injection and feeds this information back to the control unit, which automatically adjusts the time interval between injections to compensate for fluctuations, thereby maintaining chromatogram reproducibility while preserving high throughput
Solution Approach 2:
The injection time interval is changed from a fixed static value to a dynamic value that automatically adjusts based on real-time retention time measurements, allowing the system to adapt to temperature and pressure variations while maintaining separation quality
2Productivity
If the same sample is injected multiple times at regular intervals, then fractionation collecting efficiency improves, but peak overlap occurs when retention time delay increases
Solution Approach 1:
The system uses real-time retention time feedback to dynamically adjust injection intervals, ensuring that peaks from consecutive injections remain separated even during extended stack injection sequences, preventing peak overlap while maintaining high fractionation efficiency
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively suppresses the decrease in chromatogram reproducibility by continuously correcting fractionation conditions, ensuring consistent separation and recovery of components.
Implementation Method 1
A preparative liquid chromatograph (hereinafter, preparative LC) and a preparative supercritical fluid chromatograph (hereinafter, preparative SFC) is a device that separates a plurality of components in a sample from each other through chromatography using a liquid or a supercritical fluid
Implementation Method 2
a detector provided downstream of the separation column for obtaining a chromatogram of an eluate from the separation column
Implementation Method 3
a liquid feeding pump that feeds a mobile phase
Data Source
AI summary
A preparative chromatograph system (1) includes a fractionation collecting condition correction part (18) configured to detect a change in a chromatogram acquired in each of a plurality of injections of a sample during stack injection fractionation collecting and to correct a fractionation collecting condition during the stack injection fractionation collecting so that the chromatogram approaches a state before the change, wherein the stack injection fractionation collecting continues based on the corrected fractionation collecting condition after the fractionation collecting condition correction part (18) has corrected the fractionation collecting condition.