Chromatography Light Absorption Correction via Segmentation
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Solution Overview
Problem
Chromatography faces challenges in accurately correcting light absorption values, especially when using a variable eluent profile, leading to potential loss of compound peaks and introduction of false artefact peaks due to solvent composition changes.
Innovation Solution
A method that involves acquiring and using light absorption correction factors for each wavelength section and moment of the chromatographic run, allowing for individual correction of eluate light absorption values to improve peak identification and reduce artefacts.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If baseline correction is performed using derivative method to remove solvent absorption, then solvent signals are reduced, but compound peaks may be lost and false artefact peaks are introduced
Solution Approach 1:
The patent divides the light absorption spectrum into multiple wavelength sections and performs baseline correction independently for each section. This segmentation allows the derivative method to be applied locally rather than globally, preventing the loss of compound peaks and introduction of artefact peaks that occur when a single global correction is applied to the entire spectrum.
Solution Approach 2:
The patent applies different correction strategies to different wavelength sections based on their local characteristics. In regions where solvent absorption is dominant, derivative-based correction is applied. In regions where compound peaks are present, the correction is adjusted or omitted to preserve peak integrity. This local quality approach ensures that each wavelength section is corrected according to its specific needs rather than applying a uniform correction across all wavelengths.
2Productivity
If gradient eluent profile is used to improve separation, then compound separation is enhanced, but baseline correction becomes more difficult due to changing solvent composition
Solution Approach 1:
The patent segments the chromatographic run into multiple wavelength sections and applies baseline correction independently to each section. This allows the system to handle the changing solvent composition in gradient elution by treating each wavelength section as a separate problem, simplifying the overall correction process despite the complexity of the gradient profile.
Solution Approach 2:
The patent employs dynamic baseline correction that adapts to changing solvent composition throughout the gradient elution. The derivative-based correction method automatically adjusts to the local baseline conditions at each moment of the chromatographic run, allowing the system to maintain accurate correction despite the dynamic changes in eluent composition.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the accuracy of light absorption corrections, enabling better peak identification and fractionation by minimizing errors from solvent composition variations, while avoiding the loss of weak peaks and artefact introduction.
Implementation Method 1
the eluted compounds are then detected by use of a light absorption technique, primarily ultra violet light absorption
Data Source
AI summary
The present invention relates to a computer program, an apparatus and a method for correcting light absorption values measured for an eluate in connection with performing chromatography on a chemical sample, the sample being eluted with an eluent having an eluent profile with changing solvent composition during the elution of the sample.