Chromatography Column Coupling With Spring-Loaded Leak-Tight Sealing

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Solution Overview

Problem

The existing methods for connecting and disconnecting chromatographic columns in liquid chromatography systems are inefficient, requiring manual handling and tools, leading to wear and tear, increased risk of breakage, and reduced reusability due to excessive force needed for sealing.

Innovation Solution

A device and method using spring forces to securely attach and detach chromatography columns from tubing lines, ensuring a leak-tight seal without the need for manual tools, by applying separate forces to the tubing and a deformable sealing member, facilitated by a pushing and latching mechanism.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If manual handling with tools is used to connect and disconnect chromatographic columns, then the connection can be securely made, but the process is time-consuming and causes wear and tear on components

Engineering Contradiction:
Improveconnection securityVSAvoidconnection time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The system uses self-actuating mechanisms where the column and tubing automatically engage and disengage through spring-loaded sealing members and complementary geometric features, eliminating the need for manual tool operation while maintaining secure connections

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The connection mechanism incorporates movable components such as spring-loaded sealing members that dynamically adjust during the connection process to ensure proper sealing and engagement, enabling rapid yet secure coupling without manual intervention

Inventive Principle:
Principle #15Dynamics

2Reliability

If excessive force is applied to ensure a positive seal, then the seal integrity is improved, but the component lifespan is reduced due to material fatigue

Engineering Contradiction:
Improveseal integrityVSAvoidcomponent lifespan
Core Design Contradiction:
ReliabilityVSDuration of action of stationary object

Solution Approach 1:

The sealing force is concentrated at specific localized contact points between complementary geometric features of the column and tubing, rather than distributing excessive force across the entire assembly, achieving adequate seal integrity while minimizing overall stress on components

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The system uses spring-loaded sealing members that provide controlled, elastic deformation to achieve sealing, replacing the need for excessive compressive force with a more gentle elastic mechanism that reduces material fatigue

Inventive Principle:
Principle #35Parameter changes

3Reliability

If manual assembly with multiple steps is used, then the connection can be properly made, but the complexity of the procedure increases

Engineering Contradiction:
Improveconnection qualityVSAvoidassembly procedure complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

Multiple functions including alignment, sealing, and mechanical coupling are merged into a single integrated action where the column and tubing engage in one motion, eliminating the need for separate steps of assembling compression fittings and ferrules

Inventive Principle:
Principle #5Merging (Combining)

Solution Approach 2:

The system features self-aligning geometric configurations where the column and tubing automatically position themselves correctly during insertion, eliminating the need for manual alignment and positioning steps

Inventive Principle:
Principle #25Self-service

4Adaptability or versatility

If repeated manual handling is performed, then column replacement is achieved, but the risk of breakage and galling increases

Engineering Contradiction:
Improvecolumn replacement capabilityVSAvoidcomponent integrity
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The rapid exchange mechanism allows columns to be automatically inserted and removed without repeated manual handling, with the self-actuating system minimizing contact and manipulation steps that could cause galling or breakage

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The mechanism uses dynamic engagement and disengagement motions that reduce stress concentrations and prevent galling, allowing frequent column replacements while maintaining component integrity

Inventive Principle:
Principle #15Dynamics

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Enables rapid, tool-free, and reproducible connections and disconnections of chromatography columns, extending the lifespan of components and maintaining high-pressure sealing integrity, reducing material fatigue and the risk of breakage.

Implementation Method 1

a first spring within the at least one chamber, the first spring configured so as to apply a first force to the length of tubing towards the column end fitting

Methodology Applied
Scientific EffectSpring force: Spring

Implementation Method 2

a second spring within the second chamber, the second spring configured to apply a second force to a deformable sealing member towards the column end fitting

Methodology Applied
Scientific EffectSpring force: Spring

Implementation Method 3

a deformable sealing member towards the column end fitting

Methodology Applied
Scientific EffectDeformation: Deformation

Data Source

PatentEP3128322B1Apparatus for coupling two tubings to a chromatographic column
Publication Date: 2023.09.06 THERMO FINNIGAN LLC
  • EP3128322B1 patent drawingFigure 1
  • EP3128322B1 patent drawingFigure 2
  • EP3128322B1 patent drawingFigure 3A

AI summary

An apparatus for coupling a liquid chromatography column comprising an end fitting to a tubing comprises: at least one body member comprising at least one chamber; a first spring within the at least one chamber, the first spring configured so as to apply a first force to the tubing towards the column end fitting; a second spring within the at least one chamber, the second spring configured to apply a second force to a deformable sealing member towards the column end fitting; and a pushing and latching mechanism configured to push the at least one body member and the first and second springs towards the column end fitting.