Chromatography Control Device Compensating Fluid Volume Artifacts
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Solution Overview
Problem
Two-dimensional liquid chromatography apparatuses are prone to artifacts in chromatograms due to differences in flow-through properties between fluid accommodation volumes, leading to inaccuracies in sample separation results.
Innovation Solution
A control device is implemented to compensate for the different flow-through properties of fluid accommodation volumes by adjusting the operation of the sample separation apparatus, including timing adjustments for fluid flow and mobile phase profiles, and data correction methods to eliminate or suppress artifacts.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If two-dimensional liquid chromatography is used for sample separation, then separation capability is improved, but artifacts appear in chromatograms due to different flow-through properties of fluid accommodation volumes
Solution Approach 1:
The system performs preliminary characterization of fluid accommodation volumes to determine their flow-through properties before separation operations. Compensation parameters are calculated in advance based on these properties, allowing the control device to pre-adjust timing and flow rates to counteract the effects of different volume properties during actual separation, thereby eliminating artifacts while maintaining high separation capability
Solution Approach 2:
The control device dynamically adjusts operational parameters including timing of fluid flow, mobile phase profiles, and flow rates based on the characterized flow-through properties of different fluid accommodation volumes. By changing these parameters in real-time, the system compensates for volume property differences and eliminates chromatogram artifacts while preserving the enhanced separation capability of two-dimensional chromatography
2Adaptability or versatility
If fluid accommodation volumes with different flow-through properties are used in the apparatus, then operational flexibility is improved, but sample separation artifacts occur
Solution Approach 1:
The system incorporates feedback mechanisms where the control device continuously monitors separation operations and adjusts timing and flow parameters based on the known flow-through properties of different fluid accommodation volumes. This closed-loop control ensures that even when different volumes are used for operational flexibility, the system compensates for their property differences in real-time, preventing artifact generation
Solution Approach 2:
The flow-through properties of different fluid accommodation volumes are characterized and stored in advance. Before performing separations with different volumes, the control device retrieves the appropriate compensation parameters and configures the operational settings accordingly, ensuring that artifacts are prevented before they can occur while maintaining the ability to switch between different volume configurations
Data Source
AI summary
A control device for controlling at least part of a sample separation apparatus for separating a fluidic sample, the sample separation apparatus including at least two fluid accommodation volumes having different flow through properties and each being configured for temporarily accommodating fluidic sample, wherein the control device is configured for controlling operation of at least part of the sample separation apparatus for at least partially compensating sample separation artifacts resulting from the different flow through properties of the fluid accommodation volumes.