Chromatography Filter Quantification for Overlapping Peaks
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Solution Overview
Problem
Existing methods require long separation times to accurately quantify specific components in a mixture to avoid peak overlap, hindering high-speed analysis.
Innovation Solution
A method involving chromatography that applies a filter to extract a specific component's index value at each time point, generating a chromatogram for accurate quantification, and using an analysis system with a measuring instrument and analyzer to process the sample.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If chromatography is performed with low mobile phase flow rate to achieve long separation time, then measurement precision is improved, but productivity deteriorates
Solution Approach 1:
The patent applies a filter (developed from reference chromatogram data) to the measurement chromatogram before quantification. This preliminary filtering action removes interference from overlapping peaks, enabling accurate quantification even when components are not fully separated. As a result, high-speed chromatography (high flow rate) can be used without sacrificing measurement precision, thus resolving the contradiction between productivity and measurement precision.
2Productivity
If chromatography is performed with high mobile phase flow rate to achieve short separation time, then productivity is improved, but measurement precision deteriorates due to peak overlap
Solution Approach 1:
The patent introduces a filter as an intermediary processing step between chromatography and quantification. The filter, generated from reference chromatogram data, acts as a mediator that selectively enhances the target component signal while suppressing interference from other components. This allows high-speed chromatography to maintain measurement precision by computationally separating overlapping peaks, thus resolving the contradiction between productivity and measurement precision.
3Measurement precision
If long separation time is used to prevent peak overlap, then measurement precision is improved, but loss of time increases
Solution Approach 1:
The patent replaces the mechanical separation approach (relying on physical chromatographic separation over long time) with a computational approach (applying a filter algorithm to the chromatogram). Instead of extending separation time to prevent peak overlap, the filter computationally resolves overlapping peaks, thereby eliminating the need for long separation times while maintaining measurement precision, thus resolving the contradiction between measurement precision and loss of time.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high-speed and accurate quantification of specific components by applying a filter to chromatograms, allowing for rapid analysis without lengthy separation processes.
Implementation Method 1
obtaining a measurement spectrum at each of a plurality of points in time by analyzing the measurement sample with chromatography
Data Source
AI summary
Provided is a method for quantifying a specific component contained in a measurement sample. The method includes: obtaining a measurement spectrum at each of a plurality of points in time by analyzing the measurement sample with chromatography; deriving an index value at each point of the plurality of points in time, by applying a filter for extracting the specific component, to the measurement spectrum at the point of the plurality of points in time; obtaining a chromatogram by arranging one or more index values at respective one or ones of the plurality of points in time; and quantifying the specific component based on a peak of the chromatogram.