2D Chromatography Interface Module Fraction Collection
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Solution Overview
Problem
Conventional two-dimensional chromatography systems face issues with sample instability and decomposition due to multiple valves and sample loops, incompatibility of fractions between systems, pressure pulses, variability in sample loop volumes, and limited capacity for fraction collection, which affect the accuracy and lifetime of chromatographic analysis.
Innovation Solution
An interface module comprising a valve module, collection needle, modifier module, and sample storage element, with an automated positioner and controller, allowing for flexible fraction collection, modification, and injection between chromatography systems, reducing manual intervention and enhancing compatibility and stability of samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple valves and sample loops are used to collect fractions from the first chromatography system, then fraction collection capability is provided, but sample stability deteriorates due to sample decomposition and instability
Solution Approach 1:
The patent removes the traditional sample loop component from the two-dimensional chromatography system. Instead of using sample loops to hold fractions, the system directly transfers fractions from the first dimension to the second dimension chromatography system, eliminating the source of sample decomposition and instability that sample loops create.
Solution Approach 2:
The patent introduces a fraction transfer module as an intermediary component between the first and second chromatography systems. This module includes a transfer needle that can selectively access different collection vials and inject fractions into the second dimension system without requiring traditional sample loops, thereby maintaining sample stability while enabling fraction collection.
2Adaptability or versatility
If traditional valve and sample loop systems are used, then fraction collection is enabled, but device complexity increases due to multiple valves and fittings
Solution Approach 1:
The patent eliminates the need for multiple valves and complex fitting arrangements by removing the sample loop subsystem entirely. The fraction transfer module uses a simplified valve configuration with a transfer needle that can access multiple collection vials without requiring the complex valve networks traditional systems need.
Solution Approach 2:
The fraction transfer module serves multiple functions: it collects fractions from the first dimension, stores them in collection vials, and injects them into the second dimension system. This multi-functional design replaces what traditionally required separate components for each function, reducing overall device complexity.
3Quantity of substance
If sample loops with fixed volume capacities are used, then fraction storage is provided, but measurement precision deteriorates due to variability in loop volumes
Solution Approach 1:
The patent replaces fixed-volume sample loops with a dynamic injection system using a transfer needle and controllable valve. This allows precise control of the fraction volume injected into the second dimension system, ensuring consistent and accurate injection volumes regardless of the collection vial size, thereby improving measurement precision.
4Productivity
If pressure pulses occur upon injection into the second chromatography system, then fraction transfer is achieved, but column lifetime and analysis accuracy deteriorate
Solution Approach 1:
The system performs preliminary actions by controlling the injection process through the transfer needle and valve module to minimize pressure pulses before they reach the second dimension column. The gradual and controlled transfer of fractions prevents sudden pressure spikes that would otherwise damage the column and compromise analysis accuracy.
Data Source
AI summary
Described are an interface module for two-dimensional chromatography and a method of performing a chromatographic separation that may use the interface module. The interface module includes a valve module, a collection needle, a modifier module and a sample storage element. The valve module has a first port configured to receive an eluent from a first chromatography system, a second port configured to provide a fraction obtained from the eluent, a third port and a fourth port. The collection needle and the modifier module are in fluidic communication with the valve module at the third and fourth ports, respectively. The modifier module includes a source of a modifier solvent. The sample storage element is in fluidic communication with the valve module and is configured to receive a volume of the fraction for injection as a sample into a second chromatography system.