Chromatography Process Simulation With ML-Predicted Binding and Diffusion

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current chromatography process simulation methods are complex, requiring extensive experimental time and expertise due to detailed physicochemical modeling, and are not well-suited for modern chromatography columns with complex properties, limiting their practical application and scalability.

Innovation Solution

A hybrid model combining a simple physics-based mass balance model with a machine learning model to predict binding and diffusion parameters, allowing for efficient simulation of chromatography processes across different geometries and conditions, using ordinary differential equations and discrete volume elements.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If detailed first-principles mechanistic models are used to describe chromatography processes, then prediction accuracy under varying conditions is improved, but model complexity and experimental time requirements increase significantly

Engineering Contradiction:
Improveprediction accuracyVSAvoidmodel complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The patent introduces machine learning models as intermediary components that bridge the gap between simple mass balance equations and complex mechanistic models. The ML models predict the kinetic parameters (k_a, k_d, K_D) that capture binding and diffusion behavior without requiring explicit physical chemistry formulations, thus achieving accurate predictions while reducing model complexity

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent replaces complex physics-based mechanistic modeling with a data-driven machine learning approach. Instead of deriving and solving detailed partial differential equations describing mass transfer and binding mechanisms, the system uses ML models trained on experimental data to directly predict chromatography behavior, substituting mechanical/physical modeling with computational learning

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If first-principles models with multiple physical effects are used, then predictive capability is improved, but the time required for coefficient identification through experimentation increases

Engineering Contradiction:
Improvepredictive capabilityVSAvoidexperimental time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent performs preliminary action by training machine learning models on experimental chromatography data before actual process simulation. The ML models are pre-trained to capture binding and diffusion characteristics, so that when predicting new processes, the system only needs to input process conditions rather than performing time-consuming coefficient identification experiments

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent creates a computational copy of experimental chromatography behavior through machine learning models. Instead of physically conducting extensive experiments to identify model coefficients, the system trains ML models on existing experimental data to create a virtual representation of chromatography behavior, which can then be used for prediction without additional experimental time

Inventive Principle:
Principle #26Copying

3Measurement precision

If complex mechanistic models describing all physical interactions are used, then accuracy is improved, but the requirement for prior expertise and characterization knowledge increases

Engineering Contradiction:
Improvemodel accuracyVSAvoidmodel construction difficulty
Core Design Contradiction:
Measurement precisionVSEase of manufacture

Solution Approach 1:

The patent replaces expensive, time-consuming expert modeling efforts with more economical machine learning approaches. The ML models are trained on experimental data and can be applied to multiple different chromatography systems without requiring expert recalibration or re-characterization for each new application, reducing the barrier to accurate modeling

Inventive Principle:
Principle #27Cheap short-living objects (Disposable)

Solution Approach 2:

The patent changes the approach from modeling all physical interactions explicitly to predicting key behavioral parameters (binding rate k_a, dissociation rate k_d, equilibrium constant K_D) through machine learning. This parameter transformation simplifies the model construction process while maintaining predictive accuracy, as the ML models automatically capture the net effect of complex physical interactions

Inventive Principle:
Principle #35Parameter changes

4Productivity

If simple mass balance models are used, then computational efficiency is improved, but the ability to capture binding and diffusion phenomena is reduced

Engineering Contradiction:
Improvecomputational efficiencyVSAvoidbinding and diffusion capture capability
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent merges the simplicity of mass balance equations with the predictive power of machine learning models. The system combines ordinary differential equations describing bulk flow with ML-based parameter predictions for binding and diffusion, creating a hybrid model that maintains computational efficiency while capturing essential chromatography phenomena through the ML-predicted parameters

Inventive Principle:
Principle #5Merging (Combining)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The hybrid model provides a stable, computationally efficient simulation that is independent of column geometry and stationary phase type, enabling rapid and accurate predictions of chromatography performance, facilitating process optimization, monitoring, and scale-up without the need for detailed physicochemical characterization.

Implementation Method 1

a binding term captures the binding of a product to the stationary phase

Methodology Applied
Scientific EffectAdsorption: Adsorption

Implementation Method 2

a diffusion term captures the diffusion of a bound product

Methodology Applied
Scientific EffectDiffusion: Diffusion

Data Source

PatentEP4610646A1Prediction, optimisation and monitoring of chromatographic processes
Publication Date: 2025.09.03 SARTORIUS STEDIM DATA ANALYTICS AB
  • EP4610646A1 patent drawingFigure 1
  • EP4610646A1 patent drawingFigure 2A
  • EP4610646A1 patent drawingFigure 2B

AI summary

The present disclosure provides methods of simulating a chromatography process, methods of monitoring a chromatography process, methods of controlling a chromatography process, and associated systems and products, which use a bulk flow mass balance model solved over one or more discrete volume elements along the chromatography unit by numerical integration, the model being an ordinary differential equations model comprising binding and diffusion terms each parameterised by a single respective parameter that is predicted by a machine learning model.