Chromatography Sensor Detects Breakthrough for Capacity Utilization
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Solution Overview
Problem
Chromatographic matrices in biotechnological and biopharmaceutical processes have limited binding capacity utilization due to batch-dependent variations and the need to avoid overloading, leading to inefficient use of expensive materials like protein A, resulting in economically unfavorable situations and reduced process efficiency.
Innovation Solution
A device comprising a first chromatography system, a sensor for detecting substances, and a second chromatography matrix connected downstream, allowing for early detection of breakthrough without diverting material for analysis, enabling more efficient utilization of the main column's capacity and reducing losses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If the chromatographic matrix is dimensioned as small as possible to maximize binding capacity utilization, then productivity and cost-effectiveness improve, but the risk of overloading increases leading to product loss or contamination
Solution Approach 1:
A sensor is integrated into the chromatographic matrix to detect substance concentrations in advance before the matrix becomes overloaded. This preliminary detection enables early warning signals that allow optimization of the loading process, ensuring high binding capacity utilization while preventing product loss or contamination by stopping loading before critical overload occurs.
2Productivity
If a sensor is integrated into the chromatographic matrix for early detection, then binding capacity utilization improves, but device complexity increases
Solution Approach 1:
The sensor serves multiple functions simultaneously: it detects substance concentrations for early warning, monitors the loading status of the chromatographic matrix, and provides data for optimizing the binding capacity utilization. This multi-functionality justifies the additional device complexity by delivering comprehensive process control and optimization capabilities in a single integrated component.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This configuration allows for over 70% utilization of the chromatography system's capacity, minimizing product loss and reducing the need for expensive chromatographic matrices, while maintaining process efficiency and cost-effectiveness.
Implementation Method 1
a sensor for detecting the substances present in the fluid
Implementation Method 2
The central step in DSP is the chromatographic separation of the substances situated in the inflow, the feedstream. This involves the binding of certain substances to a solid matrix, which can be a membrane, a particulate gel or a monolith.
Implementation Method 3
The binding capacity of chromatographic matrices both for target molecules or contaminants is limited
Data Source
AI summary
The present invention relates to a device for separating and/or isolating substances in or from a mixture with improved utilization of the capacity of chromatographic media, the device comprising a first chromatography system, a second chromatography matrix downstream of the first chromatography system, and a sensor for detecting the substances present in the fluid. Furthermore, the present invention relates to both the use of said device and a method for separating and/or isolating substances in or from a mixture in a fluid.