Chromobacterium substsugae Formulations for Corn Rootworm Larvae Control

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Solution Overview

Problem

Current methods for controlling corn rootworm larvae infestations are not effective in consistently achieving high mortality rates and reducing egg hatching and laying, particularly with existing insecticides showing variable efficacy across different life stages and species.

Innovation Solution

The use of Chromobacterium substsugae NRRL B-30655 strain or its derived compounds in formulations applied to corn seedlings to modulate corn rootworm larvae mortality, hatching rate, and egg laying, through culture, suspension, or whole cell broth, optionally with carriers or adjuvants, to enhance pest control.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing insecticides are used to control corn rootworm larvae, then some mortality effect is achieved, but the mortality rate is variable and inconsistent across different life stages and species

Engineering Contradiction:
Improvemortality rate consistencyVSAvoidpest control effectiveness
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent changes the chemical parameters of the insecticide by formulating it with specific adjuvants and carriers that enhance its biological activity. The formulation includes compounds that modify the insecticide's solubility, stability, and mode of action, resulting in more consistent and reliable mortality rates across different corn rootworm life stages and species.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent creates a composite insecticide formulation by combining the active insecticidal compound with multiple auxiliary substances including adjuvants, carriers, and stabilizers. This composite formulation works synergistically to improve reliability and consistency of pest control effectiveness across variable environmental conditions and pest life stages.

Inventive Principle:
Principle #40Composite materials

2Productivity

If higher doses of insecticides are applied to increase mortality, then pest control effectiveness improves, but the complexity of the formulation and application process increases

Engineering Contradiction:
Improvepest control effectivenessVSAvoidformulation complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent introduces adjuvants as intermediary substances that enhance the bioavailability and efficacy of the insecticide. These adjuvants act as mediators between the active compound and the pest, improving penetration and uptake, which allows for effective pest control at optimized rather than excessive doses, thereby managing formulation complexity.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Adaptability or versatility

If broad-spectrum insecticides are used to control multiple pest species, then versatility is improved, but the specificity and targeted effectiveness against corn rootworm larvae decreases

Engineering Contradiction:
Improvepest species coverageVSAvoidtargeted effectiveness
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies the principle of local quality by designing an insecticide formulation with specific properties optimized for corn rootworm larvae. The formulation includes compounds and adjuvants that create localized effects on the target pest's physiology and behavior, ensuring high targeted effectiveness while maintaining the ability to address multiple pest species through selective pressure and resistance management.

Inventive Principle:
Principle #3Local quality

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method achieves significant mortality of corn rootworm larvae, with at least 50% mortality within three days, and reduces egg laying, providing a targeted and effective biological control for corn rootworm infestations.

Implementation Method 1

Chromobacterium substsugae NRRL B-30655 has been found to be toxic to Colorado Potato Beetle larvae but not to adults when these insects were fed a diet including Chromobacterium substsugae NRRL B-30655

Methodology Applied
Scientific EffectBiological toxicity:

Data Source

PatentUS9320282B2Use of chromobacterium substugage formulations, compositions and compounds to modulate cornworm rootworm larvae infestation
Publication Date: 2016.04.26 MARRONE BIO INNOVATIONS INC
  • US9320282B2 patent drawing
  • US9320282B2 patent drawing

AI summary

Provided is the use of or compositions or formulations comprising Chromobacterium species, filtrate, supernatant, extract, pesticidally active compound or metabolite derived therefrom as an insecticide, particularly against infestation of Corn Rootworm larvae.