Chromosome Substitution Library Assembly via Doubled Haploid Selection
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Solution Overview
Problem
Plant breeding faces challenges in efficiently combining desirable traits without the random segregation of traits that occurs during meiosis, which is time-consuming and can result in aneuploidy and the need for transgenic approaches that are costly and regulatory complex.
Innovation Solution
A method for creating chromosome substitution libraries by producing Doubled Haploid (DH) lines from heterozygous starting plants, where chromosomes are identified as non-recombined, allowing for the assembly of all possible combinations of parental chromosomes without the need for transgenic suppression of meiotic recombination, thus avoiding aneuploidy and maintaining a non-GMO status.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic approaches are used to suppress meiotic recombination, then chromosome substitution libraries can be created, but the process becomes costly and regulatory complex
Solution Approach 1:
The patent extracts and eliminates the transgenic component from the chromosome substitution library creation process. By using natural meiotic recombination without transgenic suppression, the method removes the complexity and regulatory burden associated with transgenic approaches while maintaining the ability to create chromosome substitution libraries through conventional breeding techniques.
Solution Approach 2:
The method allows the natural meiotic process to serve itself without transgenic intervention. By harnessing the natural recombination and segregation that occurs during meiosis, the patent enables chromosome substitution library creation through self-organizing biological processes rather than requiring external transgenic control mechanisms.
2Ease of manufacture
If meiotic recombination is allowed to occur naturally, then the process is simpler and non-GMO, but random segregation of traits occurs which is time-consuming
Solution Approach 1:
The patent employs marker-assisted selection as a feedback mechanism to track and select for desired trait combinations across generations. By using molecular markers to monitor chromosome inheritance and trait segregation, breeders can efficiently identify and select individuals with the desired chromosome substitutions, significantly reducing the time required compared to phenotypic selection alone.
Solution Approach 2:
The method performs preliminary chromosome substitution in the parental generation before meiotic recombination occurs. By creating parents with specific chromosome substitutions and then allowing controlled recombination, the patent prepares the genetic material in advance, reducing the number of generations needed to achieve the desired trait combinations in the final population.
3Reliability
If multiple generations are used to combine traits, then desired trait combinations can be achieved, but the process becomes time-consuming and may result in aneuploidy
Solution Approach 1:
The patent performs preliminary chromosome substitution and validation in early generations using marker-assisted selection. By establishing correctly substituted chromosome lines early in the breeding process and validating them through molecular markers, the method reduces the number of generations needed for subsequent trait combination, thereby reducing overall breeding duration while maintaining accuracy.
Solution Approach 2:
The patent replaces traditional phenotypic selection mechanics with molecular marker-based selection. This substitution allows for more precise and rapid identification of desired chromosome combinations, reducing the time required for multiple generations of selection while ensuring higher accuracy in achieving the desired trait combinations and reducing the risk of aneuploidy.
Data Source
AI summary
The invention relates to a method for producing a chromosome substitution library, comprising: a) producing a population of DH lines from an at least partially heterozygous starting organism; b) identifying a first DH line in the population that has at least one non-recombined chromosome pair; c) identifying a matching second DH line in the population that has at least one non-recombined chromosome pair that is the same as the non-recombined chromosome pair of the first DH line, and at least one non-recombined chromosome pair that is different from the non-recombined chromosome pair of the first DH line; d) selecting the first DH line and the second DH line as a pair of parents, the progeny of which has at least three non-recombined chromosomes, at least two of which form a pair; e) repeating steps b), c) and d) one or more times on the DH population of the heterozygous starting organism, or repeating steps a)-d) on a DH population produced from a progeny plant of a pair of parents selected in step d), until preferably all chromosomes from the selected DH lines are non-recombined and preferably all possible combinations of the parental chromosomes are selected, to assemble a library of chromosome substitution lines. The invention further relates to the libraries thus obtained.