Chrysanthemum White Rust Resistance Using Linked Molecular Markers

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Solution Overview

Problem

Existing technologies have not effectively addressed the need for durable genetic resistance to white rust in Chrysanthemum plants, leading to significant economic losses in cultivation.

Innovation Solution

Development of Chrysanthemum plants with genomic regions or genes providing resistance to white rust, represented by SEQ ID Nos. 1, 2, and 3, which are genetically linked and present in the plant's genome, along with molecular markers for identification.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If preventive spraying with fungicides is used, then white rust disease control is improved, but cultivation cost increases

Engineering Contradiction:
Improvewhite rust disease controlVSAvoidcultivation cost
Core Design Contradiction:
ReliabilityVSQuantity of substance

Solution Approach 1:

The patent applies preliminary action by developing and deploying molecular markers (SEQ ID Nos. 1, 2, and 3) that enable early identification of white rust resistance genes in Chrysanthemum plants. This allows breeders to select resistant plants at the seedling stage through DNA analysis rather than waiting for disease symptoms to appear, thereby pre-establishing resistance before field deployment and eliminating the need for ongoing fungicide applications.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces the mechanical/chemical system of fungicide spraying with a biological genetic system. By using molecular markers to identify and propagate plants with inherent genetic resistance (through natural resistance genes), the solution substitutes chemical disease control with genetically determined resistance, eliminating recurring chemical input costs while maintaining reliable disease control.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Quantity of substance

If susceptible Chrysanthemum varieties are cultivated, then cultivation cost is reduced, but disease susceptibility increases

Engineering Contradiction:
Improvecultivation costVSAvoidwhite rust disease susceptibility
Core Design Contradiction:
Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent introduces molecular markers (SEQ ID Nos. 1, 2, and 3) as intermediaries that bridge the gap between genetic resistance and phenotypic expression. These markers serve as detectable indicators that allow breeders and growers to identify resistant plants through simple DNA analysis, making the invisible genetic resistance visible and selectable. This intermediary enables the deployment of resistant varieties without requiring expensive chemical treatments.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Reliability

If new resistance genes are developed, then durable white rust resistance is improved, but breeding complexity increases

Engineering Contradiction:
Improvedurable white rust resistanceVSAvoidbreeding process complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts the complexity of resistance gene identification and validation by isolating specific molecular markers (SEQ ID Nos. 1, 2, and 3) that are directly linked to white rust resistance. Instead of analyzing entire genomes or conducting complex multi-generational breeding trials, the invention extracts and utilizes these specific marker sequences as reliable indicators of resistance, dramatically simplifying the breeding process while ensuring durable resistance.

Inventive Principle:
Principle #2Taking out (Extraction)

Data Source

PatentEP3522699B1White rust resistant chrysanthemum plants
Publication Date: 2025.12.10 DUEMMEN GROUP BV
  • EP3522699B1 patent drawingFigure 1
  • EP3522699B1 patent drawingFigure 2
  • EP3522699B1 patent drawingFigure 3

AI summary

The present invention relates to white rust resistant plants of the genus Chrysanthemum and to seeds, plant parts, plant cells and progeny thereof. The present invention further relates to means, and particularly molecular markers for identifying white rust resistant plants of the genus Chrysanthemum. Specifically, the present invention relates to plants belonging to the genus Chrysanthemum, the plants are resistant to white rust and the plants comprise in their genome at least one genomic region, or gene or allele, providing white rust resistance, the at least one genomic region, or gene or allele, providing white rust resistance is genetically linked to a nucleic acid sequence comprised in at least one copy in the genome of the resistant plants and is represented by SEQ ID No. 3