Chryseobacterium Microbial Compositions for Insect Resistance Control

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Solution Overview

Problem

Insect resistance to insecticides is a growing problem, necessitating the development of new insect control agents that can inhibit insecticide-resistant insects and prevent the development of resistance, particularly for Dipteran insects like Aedes mosquitoes that carry human diseases.

Innovation Solution

Compositions comprising Chryseobacterium organisms, including strains PLU6 and variants, which produce insecticidal compounds such as diindolylalkanes, are used to inhibit insect growth, reproduction, and feeding, and can be applied to plants or soil to reduce insect damage.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional insecticides are used to control insects, then insect mortality is improved, but insect resistance develops over time

Engineering Contradiction:

Improveinsect control effectivenessVSAvoidinsect resistance

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent employs multiple insecticidal compounds with distinct modes of action (different biochemical parameters) including diindolylalkanes, indole alkaloids, and other metabolites. By changing the parameter of mode of action rather than relying on a single mechanism, the composition effectively controls insects while preventing resistance development that occurs with conventional single-mode insecticides

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The invention uses a composite microbial composition containing Chryseobacterium species that produce multiple different insecticidal compounds simultaneously. This composite approach combines several insecticidal agents with different mechanisms of action in one formulation, achieving both high effectiveness and resistance prevention by attacking insects through multiple pathways at once

Inventive Principle:

Principle #40Composite materials

2Reliability

If new insect control agents are developed to overcome resistance, then insect control effectiveness is improved, but development of resistance to new agents may occur

Engineering Contradiction:

Improveinsect control effectivenessVSAvoidresistance development time

Core Design Contradiction:

ReliabilityVSDuration of action of moving object

Solution Approach 1:

The patent takes preliminary action by using a multi-compound composition from the outset rather than deploying single-mode insecticides. The Chryseobacterium-derived composition contains multiple insecticidal compounds that work through different mechanisms simultaneously, preventing resistance from developing in the first place rather than waiting for resistance to emerge and then switching agents

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The composition provides continuous effective control by maintaining multiple active compounds with different modes of action throughout the treatment period. This continuity of diverse biochemical action prevents the interruption and selection pressure that would otherwise allow resistance to develop over time

Inventive Principle:

Principle #20Continuity of useful action

Data Source

PatentUS12376589B2Chryseobacterium insect inhibitory microbial compositions and methods of making and using

Publication Date: 2025.08.05 PLUTON BIOSCIENCES INC

AI summary

Insect inhibitory compositions comprising certain Chryseobacterium organisms, compositions and compounds derived from Chryseobacterium organisms, methods of using the compositions to inhibit insects that are injurious to humans, animals, and plants, and methods of making the compositions are disclosed.