CJM01 Microalgae DHA Production via Gamma Irradiation
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Solution Overview
Problem
Current genetic transformation technologies in Thraustochytrium microalgae for producing docosahexaenoic acid (DHA) have limitations in gene copy number and expression control, and existing methods for increasing DHA productivity are not efficient.
Innovation Solution
Development of CJM01 microalgae by mutating KC01 strains through gamma ray irradiation, resulting in increased DHA production and reduced amino acid production, with a method for culturing and recovering DHA-rich biomass and bio-oil.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If chromosomal integration method is used for genetic transformation, then genes are stably maintained, but gene copy number and expression control are limited
Solution Approach 1:
The patent uses a plasmid vector as an intermediary carrier to introduce foreign genes into Thraustochytrium microalgae. This plasmid-based system serves as a mediator between the external DNA and the host genome, enabling both stable maintenance through plasmid replication origins and flexible expression control through selectable markers and promoter regions, thus resolving the contradiction between stability and productivity.
Solution Approach 2:
The patent employs selectable markers with different selection pressures (antibiotic resistance genes) to control gene expression levels and copy numbers. By changing the selection parameters during transformation and cultivation, researchers can optimize both the stability of gene maintenance and the productivity of DHA expression, addressing the technical contradiction through parameter optimization.
2Quantity of substance
If conventional microalgae strains are used, then amino acids are produced, but DHA productivity is insufficient
Solution Approach 1:
The patent extracts and introduces specific functional genes (such as fatty acid desaturase genes and elongase genes) that are responsible for DHA biosynthesis from other organisms into the Thraustochytrium microalgae. This extraction of key functional elements and their targeted insertion enables the microalgae to produce high levels of DHA without being limited by the natural metabolic pathways that prioritize amino acid production.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
CJM01 microalgae exhibit enhanced DHA productivity and reduced amino acid production, making them suitable for producing high-value DHA-rich biomass and bio-oil, addressing the limitations of existing methods.
Implementation Method 1
Development of CJM01 microalgae by mutating KC01 strains through gamma ray irradiation
Data Source
AI summary
The present disclosure relates to strains of Thraustochytrium genus, including a high content of polyunsaturated fatty acids, and a method of producing a biomass using the same. According to the novel CJM01 microalgae of Thraustochytrium genus of the present disclosure, the content of lipids in the biomass and the content of unsaturated fatty acid such as docosahexaenoic acid in the biomass are high, so that the microalgae itself, a biomass produced by the culturing and fermentation of microalgae, a condensate of the biomass, and a dried product of the biomass are very useful as a feed composition.