Cleavable Linker Reagent for Simultaneous Cell Enrichment and Staining
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Solution Overview
Problem
Current methods for preparing cell samples using antibody-modified magnetic beads require separate steps for cell enrichment and staining, leading to prolonged processing times and potential viability loss.
Innovation Solution
A reagent kit containing a solid support, a trapping substance linked by a cleavable linker, and a fluorescent dye bound closer to the trapping substance than the cleavage position, allowing for simultaneous enrichment and staining of target cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If cell enrichment and staining are performed in separate steps using antibody-modified magnetic beads, then cell enrichment can be achieved, but the processing time is prolonged and cell viability decreases
Solution Approach 1:
The patent combines cell enrichment and staining into a single integrated step by incorporating fluorescent dyes directly onto the magnetic beads that are used for cell enrichment. This allows both functions to be performed simultaneously rather than sequentially, reducing processing time and improving cell viability while maintaining enrichment efficiency.
Solution Approach 2:
The magnetic beads are designed to perform multiple functions: they serve as both the enrichment agent (through antibody coating) and the staining agent (through fluorescent dye incorporation). This multi-functional design eliminates the need for separate staining steps and enables simultaneous enrichment and visualization of target cells.
2Ease of manufacture
If cell enrichment and staining are performed in separate steps, then each step can be optimized independently, but the overall process complexity increases
Solution Approach 1:
The patent merges two separate processing steps (enrichment and staining) into a single step by integrating the fluorescent dye onto the enrichment beads. This reduces the number of processing steps from two to one, simplifying the overall process while maintaining the ability to optimize parameters during bead manufacturing.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces the time required for preparing cell samples, minimizes viability loss, and enables efficient separation of target cells using a cell sorter.
Implementation Method 1
a fluorescent dye is bound to a site on the linker closer to the trapping substance than a cleavage position in the linker or a site on the trapping substance
Data Source
AI summary
Disclosed is a reagent for preparing a cell sample, the reagent containing a solid support and a trapping substance that is linked to the solid support by a cleavable linker and traps a target cell. In the reagent for preparing a cell sample, a fluorescent dye is bound to a site on the linker closer to the trapping substance than the cleavage position in the linker, or a site on the trapping substance except for a binding site to the target cell in the trapping substance. The reagent for preparing a cell sample is useful for enriching a target cell using an antibody-modified solid support and subsequent staining for separating by a cell sorter, at the same time.