Closed Biosample Apparatus for Aseptic Cell Culture
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Solution Overview
Problem
Current cell culture technologies face challenges in efficiently isolating target cells while maintaining aseptic conditions and ensuring compatibility between target cells and culture components, leading to potential contamination and rejection issues.
Innovation Solution
A biosample processing apparatus with a closed system maintaining positive pressure to prevent external contamination, featuring a sample supply part, cell capturing part, and separation components that collect and separate target cells and blood plasma components, ensuring aseptic conditions and compatibility by using a control apparatus and drive mechanism to manage the flow of samples and reagents.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a closed system with positive pressure is used to maintain aseptic conditions, then contamination risk is reduced, but device complexity increases
Solution Approach 1:
The patent applies the inert atmosphere principle by maintaining a positive pressure environment within the closed system that prevents external contamination. The system creates a controlled atmospheric condition where the internal pressure is higher than external pressure, preventing unsterilized air from entering through any potential openings or seals during the cell culture process.
2Quantity of substance
If substances from other animal species are added to culture media, then essential components for cell culture are provided, but compatibility issues and rejection risks increase
Solution Approach 1:
The patent applies parameter changes by modifying the source and composition parameters of the culture media components. Instead of using substances from other animal species, the system uses culture media components derived from the same subject as the target cells, changing the biological compatibility parameter while maintaining the essential nutritional components needed for cell culture.
3Ease of operation
If manual cell isolation and culture media preparation are performed, then flexibility in processing is maintained, but contamination risk and processing time increase
Solution Approach 1:
The patent applies the self-service principle by designing a system where the subject's own biosample automatically provides both the target cells and the necessary culture media components. The separation unit automatically isolates target cells while the same biosample serves as the source for culture media, eliminating the need for external substances and reducing manual intervention that could introduce contamination.
Data Source
AI summary
A biosample processing apparatus of an embodiment includes a sample supply part, a first collection part, a separation part, and a second collection part. The sample supply part is connected to one end of a tubular flow path and supplies a first sample containing components extracted from a subject to the flow path. The first collection part is provided on the flow path and collects target cells to be cultured from the first sample supplied to the flow path. The separation part is provided downstream of the first collection part on the flow path and separates a blood plasma component or a blood serum component as a second sample from the first sample from which the target cells have been collected by the first collection part. The second collection part is connected to another end of the flow path and collects the second sample separated by the separation part.