Closed Cell Isolation Device for Sterile MSC Processing
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for isolating and storing mesenchymal stem cells (MSCs) from adipose tissue face challenges in maintaining sterility and viability due to the need for multiple containers and steps, which can introduce contamination and limit long-term storage capabilities.
Innovation Solution
A device comprising a cell isolation chamber and a detachable delivery unit, allowing for all processing steps, including isolation, enrichment, collection, and storage, to be performed in a single closed and sterile container assembly, enabling direct in vivo or in vitro delivery and cryogenic storage without further manipulation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If multiple containers and steps are used for cell isolation and storage, then cell processing can be completed, but sterility is compromised due to potential contamination between transfers
Solution Approach 1:
The patent combines multiple functions (isolation, enrichment, collection, and storage) into a single integrated device. The isolation chamber and delivery unit form one continuous sterile system, eliminating the need to transfer cells between multiple containers and maintaining sterility throughout the entire process.
Solution Approach 2:
The single device performs multiple functions: it isolates cells from tissue, enriches the cell population, collects the isolated cells, and provides cryogenic storage capability. This multi-functional approach replaces the traditional multi-container workflow while maintaining continuous sterility.
2Reliability
If multiple transfer steps are performed, then cell isolation can be completed, but cell viability decreases due to manipulation and contamination risk
Solution Approach 1:
The device merges the isolation chamber and delivery unit into one continuous system, allowing cells to remain in the same sterile environment throughout all processing steps. This eliminates repeated manipulation and transfer operations that would otherwise compromise cell viability.
Solution Approach 2:
The device is pre-configured with all necessary components (isolation chamber, delivery unit, cryogenic storage capability) before the procedure begins. This preliminary setup ensures that no additional manipulation or transfer steps are needed during the actual cell processing, preserving cell viability.
3Reliability
If a single closed container system is used, then sterility is maintained, but device complexity increases
Solution Approach 1:
The device is divided into distinct but integrated segments: an isolation chamber for cell separation and a delivery unit for collection and storage. These segments are connected in a way that maintains continuous sterility while allowing independent optimization of each functional area.
Solution Approach 2:
The delivery unit is positioned within or adjacent to the isolation chamber in a nested configuration, allowing both functions to operate within a single closed system. The cryogenic storage capability is integrated into the delivery unit, creating a compact nested structure that maintains sterility while managing complexity.
4Productivity
If traditional multi-step processing is used, then flexibility in handling is maintained, but time consumption increases due to multiple transfers
Solution Approach 1:
By combining isolation, enrichment, collection, and storage functions into one device, the patent eliminates the time required for transferring cells between multiple containers. All processing occurs in a single continuous workflow within the same sterile environment.
Solution Approach 2:
The device enables continuous processing from tissue input to cell storage without interruption or transfer steps. Cells flow continuously through the isolation chamber and into the delivery unit, maintaining uninterrupted useful action throughout the entire process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution ensures the sterility, viability, and functionality of MSCs, allowing for immediate clinical use or long-term storage without contamination, enhancing the efficiency and safety of cell processing and storage.
Implementation Method 1
the container unit is adapted to allow the cells isolated from the tissue to gravitationally collect in the detachable delivery unit
Data Source
AI summary
The invention generally provides a device and a method for isolating cells from tissues and using same in vivo or in vitro.