CMV Recombinant Vector With 2xFLAG/6xHIS Tags for Plant Expression
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Solution Overview
Problem
Existing gene delivery vectors for plants are not efficient in expressing foreign proteins, necessitating the development of a more effective vector for genetic manipulation.
Innovation Solution
A cucumber mosaic virus (CMV) recombinant vector with a nucleotide sequence (SEQ ID NO: 1) is developed, incorporating a multi-cloning site, 2xFLAG tag, and 6xHIS tag for enhanced protein expression, and a transgenic microorganism is transformed with this vector to increase foreign protein production in plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If existing gene delivery vectors are used for plant transformation, then the transformation process can be completed, but the foreign protein expression efficiency is low
Solution Approach 1:
The patent modifies the viral vector parameters by incorporating specific CMV genomic elements (RNA1, RNA2, RNA3 segments with specific sequences like SEQ ID NO: 1-3) and optimizing the genomic structure to enhance protein expression efficiency. This involves changing the genetic parameters of the vector to achieve higher productivity while maintaining reliability
Solution Approach 2:
The patent creates a composite viral vector system by combining multiple CMV genomic components (different RNA segments, viral proteins, and foreign gene sequences) into an integrated expression system. This composite structure leverages the synergistic effects of various viral elements to achieve high-level foreign protein expression in plants
Data Source
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AI summary
Provided is a cucumber mosaic virus-based modified recombinant vector. The recombinant vector according to an example may significantly improve the efficiency of foreign protein expression in plants, and are more efficient at expressing a foreign protein than an existing recombinant vector, and thus may be widely utilized for the production of useful proteins in various plant species and for research thereon.