CNP and HDAC Inhibitor Culture Medium for Oocyte Maturation
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Solution Overview
Problem
Current methods for in vitro maturation of oocytes are inefficient, leading to low maturation rates and reduced success in assisted reproduction technologies, both in human and livestock reproduction, due to suboptimal culture media and methods.
Innovation Solution
A culture medium comprising follicular granule cells cultured in a solution with C-type natriuretic peptide (CNP) and a histone deacetylase (HDAC) inhibitor, such as HDACi 4b, is used to promote oocyte maturation, enhancing the development potential and maturation rate of immature oocytes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional culture media are used for in vitro maturation of oocytes, then the culture process is simple, but the maturation rate and development potential of oocytes are low
Solution Approach 1:
The patent applies composite materials by combining multiple growth factors (FGF, IGF, TGF-β, VEGF, EGF) and their receptors in the culture medium to create a synergistic effect that enhances oocyte maturation. This composite approach addresses the limitation of single-factor media by integrating multiple biological activities into one system, thereby improving maturation rate without requiring multiple separate culture systems.
Solution Approach 2:
The patent implements parameter changes by optimizing the concentrations of growth factors and receptors in the culture medium. By adjusting these biochemical parameters to specific ranges, the culture medium achieves enhanced effectiveness in promoting oocyte maturation and development potential, resolving the contradiction between simplicity and effectiveness.
2Productivity
If gonadotropins are used to facilitate superovulation, then oocyte quantity increases, but the maturity of obtained oocytes decreases due to individual response differences
Solution Approach 1:
The patent uses growth factors and their receptors as intermediaries to mediate the maturation process of oocytes. These intermediaries act as signaling molecules that facilitate communication between the culture medium and oocytes, enabling enhanced maturation without relying solely on gonadotropin stimulation. This intermediary mechanism addresses the variability in individual response to gonadotropins while maintaining high oocyte quantity.
3Reliability
If in vitro maturation culture is performed to improve oocyte quality, then development potential increases, but the current culture systems are not ideal and success rates remain low
Solution Approach 1:
The patent optimizes biochemical parameters in the culture medium by incorporating specific concentrations of growth factors (FGF, IGF, TGF-β, VEGF, EGF) and their receptors. These parameter adjustments create an optimized culture environment that enhances both development potential and success rates, resolving the contradiction between quality improvement and productivity.
Solution Approach 2:
The patent employs a composite culture medium containing multiple growth factors and receptors that work synergistically to enhance oocyte maturation and developmental competence. This composite approach overcomes the limitations of conventional single-factor media, simultaneously improving development potential and success rates in assisted reproduction.
Data Source
AI summary
Provided is a method for culturing immature oocytes. The method can promote in vitro maturation of the immature oocytes, and specifically comprises using follicular cells and a culture medium for culturing same. The culture medium for culturing the follicular cells contains CNP or variants thereof or analogues thereof and an HDAC (histone deacetylase) inhibitor. Also provided are the in vitro maturation culture medium containing CNP or variants thereof or analogues thereof and the HDAC inhibitor, and related compositions thereof, and the use of the above medium, culture medium and compositions in the promotion of in vitro maturation of the immature oocytes.