Coated Magnetic Nanoparticles for Uniform Cryopreservation Rewarming
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Solution Overview
Problem
Cryopreservation by vitrification faces challenges in achieving uniform and fast rewarming of larger organs, which is essential for biobanking whole organs, as existing technologies struggle to control the warming process effectively.
Innovation Solution
Development of coated magnetic nanoparticles with a PEG-silanization coating and an additional PEG coating, which are used in a cryopreservation composition to facilitate uniform and controlled rewarming of biomaterials through an alternating magnetic field, allowing for efficient perfusion and removal of the nanoparticles from whole organs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If conventional cryopreservation methods are used, then organs can be stored, but uniform and fast rewarming cannot be achieved
Solution Approach 1:
The organ is segmented into multiple regions with independently controllable magnetic nanoparticle concentrations, allowing different rewarming rates in different regions to achieve overall uniform rewarming
Solution Approach 2:
The magnetic properties of the nanoparticles are changed by applying an alternating magnetic field, transforming them from a dormant state to an active heating state during rewarming, enabling precise control over heating parameters
2Speed
If magnetic nanoparticles are used for fast rewarming, then rewarming speed improves, but particle aggregation and instability occur
Solution Approach 1:
A composite nanoparticle structure is used with a magnetic core (for heating function) and a PEG-silanization coating (for stability), combining the advantages of both magnetic properties and colloidal stability
Solution Approach 2:
The PEG-silanization coating acts as an intermediary layer between the magnetic core and the biological environment, preventing direct interaction that would cause aggregation while allowing magnetic field penetration for heating
3Reliability
If coated magnetic nanoparticles are used, then uniform rewarming is achieved, but device complexity increases
Solution Approach 1:
The PEG-silanization coating serves multiple functions simultaneously: it provides colloidal stability, enables magnetic field penetration, facilitates uniform distribution in the organ, and prevents protein adsorption, reducing the need for additional complex components
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The coated magnetic nanoparticles enable fast and controllable heating rates, achieving uniform rewarming of whole organs and maintaining stability during cryopreservation, thereby enhancing the feasibility of cryopreservation by vitrification for biobanking.
Implementation Method 1
fast and uniform rewarming larger organs from the vitrified state is challenging. Technologies that achieve uniform, fast, controlled warming of cryopreserved organs
Implementation Method 2
a first PEG-silanization coating covalently attached to the magnetic core
Implementation Method 3
a second PEG coating covering at least a part of the first PEG-silanization coating
Data Source
AI summary
The present disclosure provides for compositions including coated magnetic particles (e.g., coated magnetic nanoparticles), methods of using the coated magnetic particles such as imaging a subject (e.g., a mammal), tissue, organ, or the like, a cryopreservation composition including the coated magnetic particles, methods of use of the cryopreservation composition in biomaterials (e.g., tissue, organ, and the like), methods of making the composition and cryopreservation composition, and the like.