COBL4 and ERF62 Proteins for Plant Drought Tolerance

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Solution Overview

Problem

Current methods fail to effectively enhance drought tolerance in plants, which is a major constraint on crop yields due to drought stress, a prevalent abiotic stress factor.

Innovation Solution

The introduction of exogenous COBL4 and ERF62 proteins or nucleic acids encoding these proteins into plants to increase their expression and activity, thereby enhancing abiotic stress tolerance, including drought tolerance, through genetic modification.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding or traditional methods are used to improve drought tolerance, then genetic diversity may be maintained, but the effectiveness and speed of enhancing drought tolerance is insufficient

Engineering Contradiction:
Improvedrought tolerance effectivenessVSAvoidtime for tolerance enhancement
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent changes the genetic parameter by introducing specific exogenous genes (COBL4 and ERF62) that encode proteins directly involved in drought stress response. This targeted genetic modification approach rapidly confers drought tolerance without requiring traditional multi-generational breeding, thus resolving the contradiction between effectiveness and time consumption.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The patent performs preliminary action by pre-selecting and introducing genes that have been proven to enhance drought tolerance based on prior research. The COBL4 and ERF62 genes are chosen because their protein products are known to play critical roles in stress response pathways, allowing the plant to acquire drought tolerance capability in advance rather than through gradual selection.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If genetic modification with exogenous proteins is implemented, then drought tolerance is enhanced, but the complexity of the genetic system increases

Engineering Contradiction:
Improveabiotic stress toleranceVSAvoidgenetic modification complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent extracts only the essential functional elements (COBL4 and ERF62 genes) needed for drought tolerance enhancement, rather than introducing complex multi-gene systems. By isolating and expressing these specific genes that directly confer stress tolerance, the patent achieves the desired reliability improvement while minimizing genetic system complexity.

Inventive Principle:
Principle #2Taking out (Extraction)

3Productivity

If current agricultural practices are maintained, then existing crop varieties can be grown, but crop yields are severely limited by drought stress

Engineering Contradiction:
Improvecrop yieldVSAvoiddrought stress impact
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effect of drought stress into a benefit by introducing genes that enable the plant to actively respond to and withstand drought conditions. The COBL4 and ERF62 proteins enhance the plant's ability to sense, signal, and respond to water deficit, transforming the previously harmful drought environment into a manageable condition that allows maintained or improved productivity.

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Data Source

PatentUS10190133B2Compositions and methods for improving abiotic stress tolerance
Publication Date: 2019.01.29 CHINA AGRI UNIV
  • US10190133B2 patent drawing
  • US10190133B2 patent drawing
  • US10190133B2 patent drawing

AI summary

The present invention relates to compositions and methods for improving the abiotic stress tolerance of plants. Plants and plant parts identified, selected and/or produced using compositions and methods of the present invention are also provided.