Fluorescent Co-Culture Imaging for Quantifying Tumor Stroma Status

The patent creates in vitro co-culture models that copy the essential biological interactions of in vivo mouse models. By culturing target cells with fibroblasts and immune cells in controlled environments, the system replicates the modulatory effects observed in living organisms without requiring animal subjects, thereby maintaining biological relevance while eliminating the time and resource costs of animal modeling.

The patent replaces the complex mechanical and physiological systems of whole animal models with simplified in vitro culture systems. By substituting the in vivo biological system with an in vitro cell culture system, the research can study modulatory effects using only the necessary cellular components, reducing complexity while preserving the core biological interactions of interest.

The patent creates in vitro co-culture models that copy the essential biological interactions of in vivo mouse models. By culturing target cells with fibroblasts and immune cells in controlled environments, the system replicates the modulatory effects observed in living organisms without requiring animal subjects, thereby maintaining biological relevance while eliminating the time and resource costs of animal modeling.

The patent employs disposable in vitro culture systems that can be easily set up and discarded after use, replacing expensive and resource-intensive animal models. The cell culture approach uses inexpensive culture media and standard laboratory equipment, making the research significantly more cost-effective while maintaining scientific validity.

The patent segments the complex biological system into discrete cellular components (target cells, fibroblasts, immune cells) that can be independently cultured and manipulated. This segmentation allows each cell type to be optimized separately and enables parallel processing of multiple samples, thereby achieving both biological complexity and high-throughput screening capability simultaneously.

The patent employs dynamic co-culture systems where cell interactions can be adjusted and controlled in real-time. The system allows for flexible modification of cell ratios, culture conditions, and treatment protocols, enabling both complex biological studies and standardized high-throughput screening by adapting the configuration to specific experimental requirements.