Coded Filter Fluorescence Imaging for Reflection Light Separation
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Solution Overview
Problem
Existing fluorescence imaging systems struggle to accurately distinguish between fluorescent light and reflection light due to overlapping wavelength spectra, leading to superimposed images and difficulties in obtaining hyperspectral images, especially when using compressed sensing technology.
Innovation Solution
An imaging apparatus employing a coding filter array with filters having distinct transmission spectra and a processing circuit to generate hyperspectral images by distributing excitation light across multiple filters, suppressing luminance saturation and enhancing image reconstruction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a coding filter array is used to capture compressed image data for hyperspectral imaging, then image reconstruction capability is improved, but luminance saturation occurs and measurement precision deteriorates
Solution Approach 1:
The patent segments the excitation light spectrum by using multiple filters with different transmission spectra in the coding filter array. Each filter captures a specific portion of the excitation light spectrum, distributing the luminance across multiple channels and preventing saturation in any single channel while maintaining comprehensive spectral information for accurate hyperspectral image reconstruction
Solution Approach 2:
The patent applies local quality by assigning different transmission spectrum characteristics to different filters within the coding filter array. Each filter is designed with specific transmission properties tailored to its function in capturing particular spectral components, enabling optimized light distribution that prevents saturation while preserving measurement precision
2Measurement precision
If excitation light spectrum overlaps with filter transmission region, then fluorescent light capture is improved, but reflection light interference increases and measurement precision deteriorates
Solution Approach 1:
The patent segments the spectral detection task across multiple filters, each with distinct transmission spectra. This segmentation allows the system to capture fluorescent light across different spectral bands while distributing reflection light interference across multiple channels, enabling effective separation and accurate fluorescent light detection through compressed sensing reconstruction
Solution Approach 2:
The patent utilizes parameter changes by varying the transmission spectrum parameters of different filters in the coding filter array. This variation in filter transmission characteristics enables the system to capture fluorescent emission spectra at multiple wavelength points while managing reflection light interference through diverse spectral filtering, improving measurement precision
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables accurate generation of hyperspectral images by effectively separating fluorescent and reflection light spectra, reducing saturation and improving signal-to-noise ratios, thereby enhancing image quality.
Implementation Method 1
When an object provided with a fluorophore is irradiated with excitation light, fluorescent light is emitted from the fluorophore
Implementation Method 2
a coding filter array including filters whose transmission spectra differ from each other
Data Source
AI summary
An imaging apparatus includes: at least one light source that emits excitation light for irradiating an object including at least one light emitter; a coding filter array including filters whose transmission spectra differ from each other; an image sensor that captures an image of object light, which is generated by irradiating the object with the excitation light, through the coding filter array and generates compressed image data; and a processing circuit that generates hyperspectral image data based on the compressed image data. The object light includes emission light, which is produced by the at least one light emitter by absorbing the excitation light, and reflection light of the excitation light reflected by the object. The filters include two filters whose transmission spectra differ from each other. A spectrum of the excitation light overlaps a transmission region in the transmission spectrum of each of the two filters.