CODH Enzyme Residue 97 Mutation for High CO Tolerance
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Solution Overview
Problem
Current technologies face challenges in developing microorganisms that can thrive and efficiently produce metabolites at high carbon monoxide concentrations, limiting their commercialization potential due to complex genetic engineering requirements and limited CO tolerance.
Innovation Solution
A protein variant of the CODH enzyme from Eubacterium limosum, where the 97th alanine residue is substituted with a different amino acid, such as glutamic acid, enhancing CO availability and tolerance in microorganisms, allowing for improved growth and metabolite production in high CO environments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If acetogens are used to consume waste gas and produce metabolites, then carbon resource utilization is improved, but growth is severely limited at high CO concentrations
Solution Approach 1:
The patent applies parameter changes by mutating the amino acid sequence of the CODH enzyme (specifically changing residue 97 from alanine to glutamic acid), which alters the enzyme's kinetic parameters and affinity for CO substrates, enabling the microorganism to thrive at high CO concentrations while maintaining metabolite production
2Adaptability or versatility
If genetic engineering is performed to enhance CO tolerance, then high CO concentration adaptability is improved, but the complexity of genetic engineering increases
Solution Approach 1:
The patent extracts and focuses on a single critical residue (residue 97) in the CODH enzyme that is responsible for CO binding affinity. By targeting only this specific residue for mutation rather than performing comprehensive genetic engineering, the complexity is reduced while still achieving significant CO concentration adaptability enhancement
3Productivity
If CO concentration is increased as a stressor for evolution, then CO availability is enhanced, but growth rate decreases
Solution Approach 1:
The patent converts the harmful effect of high CO concentration (which normally inhibits growth) into a beneficial selective pressure that drives evolution. By exposing the microorganism to high CO concentrations during evolution, a mutant strain is selected that has enhanced CO availability through residue 97 mutation, turning the previously harmful high CO environment into a condition where the mutant can thrive and produce metabolites
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The modified microorganisms exhibit enhanced CO tolerance and increased metabolite production, enabling effective utilization of CO in waste gases, thus addressing the limitations of existing technologies and enhancing carbon resource utilization.
Implementation Method 1
The recently emerging carbon resource technology is a technology that utilizes carbon monoxide, carbon dioxide, methane, natural gas, etc. generated from fossil fuels, etc. as a raw material
Data Source
AI summary
The present invention relates to a protein variant, a microorganism with enhanced carbon monoxide (CO) availability comprising the variant, and a use thereof.