Cold-Adapted Bacillus Strains for Faster Root Colonization
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Solution Overview
Problem
Existing biological control methods for plant pests, particularly using Bacillus paralicheniformis, are limited by low growth rates and biomass formation at different temperatures, which hampers effective colonization and protection of plant roots, and there is a need for strains with improved growth rates and biomass yields to enhance plant growth promotion and disease control.
Innovation Solution
Development of cold-adapted derivative strains of Bacillus paralicheniformis with increased growth rates and biomass formation at various temperatures through adaptive laboratory evolution, specifically targeting mutations in the BioF and HrcA genes to enhance colonization and biofilm formation.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional Bacillus paralicheniformis strains are used for biological control, then plant protection function is provided, but growth rates and biomass yields are low at different temperatures
Solution Approach 1:
The patent applies parameter changes by modifying specific genetic parameters (BioF and HrcA genes) to alter the physiological parameters of the bacteria. The BioF gene modification changes biotin synthesis capability, while HrcA gene modification affects heat shock protein expression, collectively improving growth rates and biomass yields across different temperature conditions, thereby resolving the contradiction between productivity and reliability.
Solution Approach 2:
The patent applies local quality by making specific localized modifications to the bacterial genome at particular gene loci (BioF and HrcA) rather than global changes. This targeted approach improves specific functions (growth rate and biomass production) while maintaining other essential bacterial characteristics, enabling enhanced productivity without compromising overall colonization efficiency.
2Quantity of substance
If conventional Bacillus paralicheniformis strains are used, then basic biocontrol function is achieved, but biomass formation and colonization ability are insufficient
Solution Approach 1:
The patent modifies genetic parameters (BioF and HrcA genes) to change physiological parameters, resulting in simultaneous improvement of both biomass yield and growth rate. The BioF gene enhancement increases biotin availability for biomass accumulation, while HrcA modification improves protein synthesis efficiency, collectively resolving the contradiction between quantity of substance and productivity.
3Adaptability or versatility
If temperature range is expanded for application, then adaptability is improved, but growth performance decreases at lower temperatures
Solution Approach 1:
The patent applies parameter changes by modifying the HrcA gene, which regulates heat shock protein expression and stress response. This genetic modification enables the bacteria to maintain optimal physiological parameters across a wider temperature range, particularly improving growth rate at lower temperatures while maintaining temperature adaptability, thus resolving the contradiction between adaptability and productivity.
Data Source
AI summary
The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.