Cold Atmospheric Plasma for IBRV Replication in MDBK Cells
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Solution Overview
Problem
Current vaccine production methods for infectious bovine rhinotracheitis virus (IBRV) face inefficiencies and high costs, necessitating a method to promote IBRV replication for improved vaccine production efficiency.
Innovation Solution
A method involving the irradiation of a medium for Madin-Darby bovine kidney cells using a cold atmospheric plasma generator, followed by addition of the irradiated medium and IBRVs for incubation, which enhances IBRV replication through the generation of reactive species in the medium.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional vaccine production methods are used, then vaccine production can be maintained at current levels, but vaccine production efficiency is low and costs are high
Solution Approach 1:
The patent applies parameter changes by treating the culture medium with cold atmospheric plasma, which modifies the physical and chemical parameters of the medium (generating reactive oxygen species, nitrogen species, and other active substances). This treatment creates a more favorable environment for viral replication, thereby increasing virus yield and improving vaccine production efficiency without changing the fundamental production process
Solution Approach 2:
The patent replaces conventional mechanical or chemical methods of enhancing virus replication with a plasma-based approach. Instead of using traditional methods such as optimizing temperature, pH, or adding chemical additives, the invention uses cold atmospheric plasma generation to create reactive species that naturally promote viral replication in the culture medium
2Quantity of substance
If plasma treatment is applied to promote IBRV replication, then virus yield increases, but the process complexity increases
Solution Approach 1:
The patent uses cold atmospheric plasma as an intermediary to transfer energy and reactive species to the culture medium. The plasma generator creates a plasma jet that interacts with the medium, generating beneficial reactive substances without requiring direct contact between the plasma source and the cells or virus. This intermediary approach simplifies the overall process while achieving the desired enhancement in virus replication
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method significantly promotes IBRV replication, resulting in higher virus yields that can be used for vaccine production, thereby improving vaccine production efficiency and reducing costs.
Implementation Method 1
irradiating a medium for Madin-Darby bovine kidney (MDBK) cells with a cold atmospheric plasma generator
Implementation Method 2
a cold atmospheric plasma generator; adding the irradiated medium to the MDBK cells
Data Source
AI summary
A method for promoting replication of infectious bovine rhinotracheitis viruses using cold atmospheric plasma, including: irradiating a medium for Madin-Darby bovine kidney cells with a cold atmospheric plasma generator; adding the irradiated medium to the Madin-Darby bovine kidney cells; and adding infectious bovine rhinotracheitis viruses for incubation. The time required for treatment is 2 min allowing for a simple and rapid operation. The plasma is used for the indirect treatment of cells with a uniform process and a controllable intensity. The replication of the infectious bovine rhinotracheitis viruses in the Madin-Darby bovine kidney cells is significantly promoted by co-incubation in the treated DMEM for 1 hour, so that the high levels of infectious bovine rhinotracheitis viruses obtained can be used for vaccine production after inactivation, improving the vaccine production efficiency.