Low-Temperature Nitrifying Bacteria Cultivation by pH Adjustment
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Solution Overview
Problem
Existing biological treatment methods for nitrogen-containing water are ineffective at low temperatures, requiring cumbersome heating equipment and large amounts of culture medium, making it difficult to culture low temperature-resistant nitrifying bacteria efficiently.
Innovation Solution
A method involving pH adjustment of nitrogen-containing water to 6.0 or lower to cultivate low temperature-resistant nitrifying bacteria, combined with a device comprising interconnected tanks for culturing and nitrification, allowing nitrification to occur at temperatures between 0°C and 15°C using these bacteria.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional biological treatment is used at low temperatures, then treatment cost is low, but the reaction does not progress and treatment cannot be carried out
Solution Approach 1:
The patent changes the pH parameter of the water to be treated to a range of 5.0 or lower, which creates favorable conditions for low temperature-resistant nitrifying bacteria to become dominant and carry out effective nitrification at low temperatures without requiring heating
2Reliability
If heating equipment is used to heat the water before biological treatment, then treatment can proceed, but the process becomes very cumbersome
Solution Approach 1:
Instead of changing temperature through heating equipment, the patent changes the pH parameter to create favorable conditions for low temperature-resistant bacteria, thereby avoiding the need for heating equipment and simplifying the treatment process
3Reliability
If AH bacteria are cultured using a large amount of culture medium containing highly-concentrated ammonia, then low temperature-resistant nitrifying bacteria can be obtained, but the culturing equipment becomes enormous
Solution Approach 1:
The patent changes the pH parameter of the water to be treated to 5.0 or lower, which naturally selects for and cultures low temperature-resistant nitrifying bacteria without requiring large amounts of specially formulated culture medium containing highly-concentrated ammonia
Solution Approach 2:
The treatment process itself serves to culture the bacteria, as the low pH condition naturally selects for low temperature-resistant nitrifying bacteria to become dominant during the nitrification process, eliminating the need for separate large-scale culturing operations
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient cultivation of low temperature-resistant nitrifying bacteria without large culture media, facilitating effective nitrification at low temperatures without heating, thus simplifying the treatment process.
Implementation Method 1
adjusting a pH of nitrogen-containing water a containing ammonia nitrogen and/or organic nitrogen to not more than 6.0 to obtain nitrogen-containing water b in which a dominant species of microorganisms is low temperature-resistant nitrifying bacteria
Implementation Method 2
produces a nitrification liquid by nitrifying, at a water temperature of not lower than 0° C. and not higher than 15° C. with use of the low temperature-resistant nitrifying bacteria contained in the nitrogen-containing water b, ammonia contained in the nitrogen-containing water a and/or the nitrogen-containing water b
Data Source
AI summary
Provided are (i) a method for culturing low temperature-resistant nitrifying bacteria by a simple method, (ii) a method for treating nitrogen-containing water with use of the low temperature-resistant nitrifying bacteria, and (iii) a device capable of culturing the low temperature-resistant nitrifying bacteria and treating nitrogen-containing water. A method for culturing low temperature-resistant nitrifying bacteria, the method including the step of: adjusting a pH of nitrogen-containing water a containing ammonia nitrogen and/or organic nitrogen to not more than 6.0 to obtain nitrogen-containing water b in which a dominant species of microorganisms is low temperature-resistant nitrifying bacteria.