High-Voltage Cold Plasma Seed Activation for Faster Mutation Breeding
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Solution Overview
Problem
The breeding of Brassica campestris ssp. chinensis Makino is hindered by a narrow genetic background and low efficiency in creating new varieties, leading to genetic vulnerability and difficulty in obtaining crops with excellent resistance to adversity and high-yielding characteristics.
Innovation Solution
A method involving the activation of crop seeds using high-voltage electric field cold plasma (HVCP) by immersing seeds in water and applying a voltage of 80 kV to 130 kV, which induces mutations and enhances genetic diversity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If hybrid breeding and male sterile lines are used for cultivation, then variety improvement is achieved, but breeding cycle is long and efficiency is low
Solution Approach 1:
The patent replaces traditional mechanical breeding methods (hybrid breeding, male sterile lines) with physical mutagenesis using high-voltage electric field cold plasma. This substitution enables direct induction of genetic mutations in seeds, bypassing the need for multi-generation hybrid breeding and dramatically shortening the breeding cycle while improving efficiency.
Solution Approach 2:
The patent applies high-voltage electric field parameters (80-130 kV) to seeds to induce mutations. By changing the physical state and energy parameters applied to seeds, new germplasms are generated directly, eliminating the time-consuming process of traditional hybrid breeding cycles.
2Productivity
If chemical mutagenesis is used to generate new germplasms, then mutation is achieved, but safety concerns arise
Solution Approach 1:
The patent substitutes chemical mutagenesis agents with physical high-voltage electric field cold plasma treatment. This replacement eliminates exposure to harmful chemical mutagens while maintaining effective mutation induction, addressing safety concerns without sacrificing mutation rate.
Solution Approach 2:
The patent converts the potentially harmful high-voltage electric field into a beneficial cold plasma state that induces mutations safely. The high voltage is transformed into a controlled plasma environment that mutagenizes seeds without the chemical hazards of traditional mutagenesis agents.
3Productivity
If intensive breeding with narrow genetic background is pursued, then high yield is achieved, but genetic vulnerability increases
Solution Approach 1:
The patent uses high-voltage electric field parameters to induce genetic variations in seeds, creating new germplasms with diversified genetic backgrounds. This parameter-based mutagenesis generates genetic diversity that enhances resistance to adversity while maintaining or improving yield potential.
Solution Approach 2:
The patent performs mutagenesis on seeds before planting, creating genetically diverse germplasms in advance. This preliminary genetic modification ensures that plants have inherent resistance to adversity from the outset, rather than relying on narrow genetic backgrounds from intensive breeding.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method improves yield and heat resistance, provides a high mutation rate, and shortens the breeding cycle, offering a more efficient and safe alternative to chemical mutagenesis.
Implementation Method 1
conducting discharge activation on the immersed crop seed at a voltage of 80 kV to 130 kV
Implementation Method 2
high-voltage electric field cold plasma (HVCP)
Data Source
AI summary
The present disclosure relates to a method for activating a crop seed by high-voltage electric field cold plasma (HVCP), and use thereof, belonging to the technical field of crop planting. The present disclosure provides a method for activating a crop seed by HVCP, including the following steps: (1) mixing a crop seed with water and immersing the crop seed in water for 4 h to 24 h to obtain an immersed crop seed; and (2) conducting discharge activation on the immersed crop seed at a voltage of 80 kV to 130 kV to obtain an activated crop seed. In the present disclosure, the method can improve heat-resistant and disease-resistant properties of crops, and provide a scientific basis for subsequent acquisition of excellent seeds.