Cold-Tolerant Bacillus Strains for Low-Temperature Phytostimulation

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Solution Overview

Problem

Current biological plant protection methods using mesophile bacteria are ineffective at temperatures below 15°C, limiting their application in cold ecosystems, despite the need for sustainable and environmentally friendly alternatives for plant growth promotion in regions like mountainous areas and those near the poles.

Innovation Solution

The use of cold-tolerant Bacillus strains, such as Bacillus atrophaeus, Bacillus simplex, and Bacillus pumilus, which can grow and promote plant growth at temperatures below 15°C, combined with mesophile bacteria and humic acids, to stimulate plant growth and inhibit pathogens in challenging climatic conditions.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If mesophile bacteria are used for plant growth promotion, then effective phytostimulatory effect is achieved, but application is limited to temperatures above 15°C

Engineering Contradiction:

Improvephytostimulatory effectVSAvoidtemperature range

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent applies parameter changes by selecting and using cold-tolerant Bacillus strains that have adapted their growth parameters to function effectively at lower temperatures (≤10°C to 0°C). These strains maintain their phytostimulatory capabilities across a broader temperature range, specifically enabling plant growth promotion in cold ecosystems where traditional mesophile bacteria fail.

Inventive Principle:

Principle #35Parameter changes

2Adaptability or versatility

If cold-tolerant bacteria are used to expand temperature range, then application in cold ecosystems becomes possible, but effectiveness at optimal growth temperatures may be reduced

Engineering Contradiction:

Improvetemperature rangeVSAvoidgrowth rate

Core Design Contradiction:

Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent employs partial action by using cold-tolerant Bacillus strains that provide sufficient phytostimulatory effect at suboptimal temperatures (≤15°C) where traditional strains are completely ineffective. While these cold-adapted strains may grow slower at optimal temperatures compared to mesophiles, they deliver adequate productivity in their target cold environment where the alternative is zero effectiveness.

Inventive Principle:

Principle #16Partial or excessive action

3Object-affected harmful factors

If biological means are used instead of chemical pesticides and fertilizers, then environmental friendliness is improved, but effectiveness at low temperatures is reduced

Engineering Contradiction:

Improveenvironmental impactVSAvoideffectiveness at low temperature

Core Design Contradiction:

Object-affected harmful factorsVSReliability

Solution Approach 1:

The patent resolves this contradiction by changing the biological parameter of the microorganisms used - selecting cold-tolerant Bacillus strains that maintain their biological activity and phytostimulatory effects at low temperatures. This ensures that the environmental benefits of biological means are preserved while overcoming the temperature limitation that previously reduced their reliability in cold ecosystems.

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS11180727B2Selection and use of cold-tolerant bacillus strains as biological phytostimulators

Publication Date: 2021.11.23 ABITEP

AI summary

The invention relates to a biological means for improving the yield of cultivated plants. The areas of application of the invention are agriculture, horticulture and plant protection.