Collagen 7 Production via Codon Optimization and Enzyme Co-Expression
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for producing collagen 7, a crucial component of anchoring fibrils, are inefficient and lack effective means to ensure proper processing and incorporation into stable structures, affecting tissue integrity and stability.
Innovation Solution
A method involving genetically modified mammalian cells, such as CHO or HEK cells, that express collagen 7 and associated enzymes like prolidase and prolyl hydroxylase, optimized for high glycine codon sequences, to enhance production and proper processing of collagen 7, facilitating its incorporation into homotrimers or hexamers.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods are used to produce collagen 7, then production can occur with standard cell lines, but the efficiency and yield are low
Solution Approach 1:
The patent optimizes nucleic acid sequences by changing codon parameters to match host cell preferences (codon optimization), and modifies cell culture parameters (co-expression of processing enzymes) to enhance collagen 7 production efficiency and yield while maintaining manufacturability
Solution Approach 2:
The patent introduces intermediary enzymes (prolidase and prolyl hydroxylase) that mediate the processing of procollagen 7 to mature collagen 7, enabling efficient production by facilitating proper structural conversion and assembly into stable fibrils
2Manufacturing precision
If standard cell lines are used without additional modifications, then the manufacturing process is simpler, but proper processing and structural assembly of collagen 7 is insufficient
Solution Approach 1:
The patent combines multiple functions into a single engineered cell line by co-expressing collagen 7 along with essential processing enzymes (prolidase and prolyl hydroxylase), ensuring proper structural assembly and processing accuracy while consolidating manufacturing steps
Solution Approach 2:
The patent performs preliminary genetic engineering of cell lines to include all necessary processing enzymes before collagen 7 production, ensuring that proper structural assembly capabilities are pre-established in the host cells
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enables efficient production and purification of collagen 7, ensuring high yields and proper structural assembly, thereby improving tissue stability and integrity.
Implementation Method 1
prolyl hydroxylase
Implementation Method 2
prolidase
Data Source
AI summary
Disclosed are methods of making collagen 7, or functional fragments thereof, as well as collagen 7, and functional fragments thereof produced by such methods, nucleic acids encoding collagen 7, and functional fragments thereof, as well as vectors and host cells comprising such nucleic acids.