Collagen-Rich Composition Extraction From Teeth and Bone

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Solution Overview

Problem

Existing collagen products lack adequate quality evaluation based on molecule-level indices, particularly pyridinoline and pentosidine content, and extraction from hard tissues like teeth and bones is labor-intensive with restricted yield, leading to insufficient assessment of potential morbidity risk and health status in mammals.

Innovation Solution

A method involving strong alkali treatment and demineralization under negative pressure to extract collagen from teeth or bones, followed by high-performance liquid chromatography to quantify pyridinoline and pentosidine content, enabling the production of a collagen-rich organic composition with enhanced elasticity and strength, and assessment of potential morbidity risk.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If demineralization treatment is performed to extract collagen from hard tissues, then collagen can be obtained, but the extraction process is labor-intensive and yield is restricted

Engineering Contradiction:

Improvecollagen yieldVSAvoidextraction labor

Core Design Contradiction:

Quantity of substanceVSEase of manufacture

Solution Approach 1:

The patent applies parameter changes by optimizing the demineralization conditions including pH control (using acetate buffer at pH 4.5-5.5), temperature conditions (4°C refrigeration), and time parameters (72-96 hours). These controlled parameter changes enable efficient collagen extraction from hard tissues while maintaining high yield and reducing manual labor requirements through standardized protocol.

Inventive Principle:

Principle #35Parameter changes

2Reliability

If enzyme treatment is performed to produce atelocollagen, then telopeptides are severed and antigenicity is reduced, but purification process removes hardly-soluble collagen fibers and reduces yield

Engineering Contradiction:

Improveantigenicity reductionVSAvoidcollagen yield

Core Design Contradiction:

ReliabilityVSQuantity of substance

Solution Approach 1:

The patent employs parameter changes by precisely controlling pH conditions during purification (maintaining pH 4.5-5.5 using acetate buffer) and temperature (4°C refrigeration). These controlled parameters enable the separation of mineral components while preserving both soluble and hardly-soluble collagen fractions, thereby maintaining high yield while achieving the desired antigenicity reduction through selective demineralization rather than aggressive enzyme treatment.

Inventive Principle:

Principle #35Parameter changes

3Strength

If pyridinoline content is increased to enhance collagen elasticity and strength, then collagen quality improves, but assessment of potential morbidity risk becomes insufficient without pentosidine content evaluation

Engineering Contradiction:

Improvecollagen elasticity and strengthVSAvoidhealth assessment accuracy

Core Design Contradiction:

StrengthVSReliability

Solution Approach 1:

The patent applies universality by developing a dual-purpose analytical system that simultaneously evaluates both pyridinoline content (indicating collagen strength and elasticity) and pentosidine content (indicating glycation and aging). This multi-functional assessment approach enables comprehensive health evaluation including both collagen quality metrics and morbidity risk assessment, making the system universally applicable for both biomaterial quality control and clinical health screening.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method produces a collagen-rich organic composition with improved steric structure, allowing for discernment of high-quality collagen and assessment of health status through pyridinoline and pentosidine ratios, reducing endotoxin content and enhancing collagen quality for biomaterials.

Implementation Method 1

giving strong alkali treatment under a condition of temperature higher than normal temperature and lower than water boiling point to a pulverization product

Methodology Applied

Scientific EffectAlkali treatment:

Implementation Method 2

giving demineralization treatment under a negative pressure condition to the pulverization product given strong alkali treatment

Methodology Applied

Scientific EffectDemineralization:

Implementation Method 3

quantifying pyridinoline content (ng/mg) of collagen contained in teeth of a mammal, or a quantification value of pentosidine content (ng/mg) thereof, by means of high-performance liquid chromatography with a fluorescence detector

Methodology Applied

Scientific EffectChromatography: Chromatography

Data Source

PatentUS20260053981A1Collagen-rich organic composition and production method thereof

Publication Date: 2026.02.26 DEVINE INC

AI summary

[Problem] To provide: a collagen-rich organic composition; a biomaterial using the collagen-rich organic composition for an ingredient material; a biomaterial or other product containing collagen as a main ingredient; a method of producing the collagen-rich organic composition; a method of discerning teeth or bones extracted from a mammal to be used as an ingredient material of a biomaterial or a biomaterial or other product containing collagen as a main ingredient mammal; and a method of using, as an indicator for assessing a potential morbidity risk of a disease in a mammal and/or whether a mammal is potentially in good health or not, PYD as well as PYD and PpP.[Solution] PYD>100 as well as PYD>100 and PpP>740.