Composite Collagen Sponge Virus Inactivation and Bioactivity
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing collagen-based biomedical materials for wound healing and tissue repair face safety risks due to potential viral contamination and instability during storage, with previous methods using radiation for disinfection that can denature bioactive materials and reduce their effectiveness.
Innovation Solution
A composite collagen sponge is developed using type I collagen from pig skin, purified through salting-out and chromatography, with a two-stage virus inactivation process involving solvent/detergent treatment and heat-based methods to ensure safety and stability, combined with the addition of protecting agents like amino acids, saccharides, and albumin to maintain bioactivity and enhance water absorption and elastic properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If radiation is used for disinfection during preparation of composite collagen sponges, then virus inactivation is achieved, but the temperature increases causing denaturation of bioactive materials and reducing their activity
Solution Approach 1:
The patent introduces an intermediary substance (collagen matrix) that allows virus inactivation through chemical or physical methods without direct thermal exposure. The collagen sponge serves as a carrier that protects incorporated bioactive materials from denaturation while enabling effective virus inactivation through alternative mechanisms such as solvent/detergent treatment or filtration methods.
Solution Approach 2:
The patent changes the inactivation parameters from high-temperature radiation to milder chemical or physical methods. By using solvent/detergent treatments or controlled chemical inactivation processes, the patent achieves virus inactivation while maintaining the temperature and chemical environment necessary for preserving bioactive material integrity and activity.
2Ease of manufacture
If collagen is extracted from animal tissues without purification, then the preparation process is simpler, but viral contamination risk increases
Solution Approach 1:
The patent applies extraction principles by selectively removing viral contaminants from the collagen matrix through purification steps. The process extracts and eliminates harmful viral particles while retaining the beneficial collagen structure and incorporated bioactive materials, achieving both safety and simplicity.
Solution Approach 2:
The patent uses composite material principles by creating a multi-component system where collagen serves as the base matrix and purification agents or antiviral compounds are integrated into the structure. This composite approach enables simultaneous achievement of viral inactivation and process simplicity through built-in safety mechanisms.
3Reliability
If bioactive materials are incorporated into collagen sponges, then wound healing and tissue repair effects are enhanced, but the product complexity and manufacturing difficulty increase
Solution Approach 1:
The patent merges the collagen matrix and bioactive materials into a single integrated sponge structure. By combining these components during the manufacturing process rather than assembling them separately, the patent reduces manufacturing steps and complexity while maintaining the enhanced wound healing effects of the bioactive materials.
Solution Approach 2:
The patent creates a multi-functional sponge that simultaneously provides structural support (collagen), therapeutic effects (bioactive materials), and virus inactivation capability. This universal design consolidates multiple functions into a single product, reducing the need for separate manufacturing processes and simplifying overall production.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composite collagen sponge achieves high stability during storage, superior water absorption, and prolonged therapeutic effects, ensuring safety and efficacy in clinical applications by effectively inactivating viruses and maintaining bioactive material activity.
Implementation Method 1
a two-stage virus inactivation process involving solvent/detergent treatment
Implementation Method 2
heat-based methods to ensure safety and stability
Implementation Method 3
purified through salting-out and chromatography
Implementation Method 4
purified through salting-out and chromatography
Data Source
Figure 1~3
AI summary
The present invention discloses a composite collagen sponge and the preparation method thereof. The composite collagen sponge comprises collagen, cell growth factors, and a protecting agent, and has a water absorption capacity of more than 52 times. The preparation method includes a cation chromatographic purification step and a two-stage inactivation step consisting of organic solvent/detergent virus inactivation and dry heat virus inactivation. The composite collagen sponge obtained by the method of the present invention not only improves the safety and performance of the product in clinical applications, but also ensures the stability and the bioactivity throughout the effective life of the product.