Colloidal Gold Test Strip for Stichopus Oligopeptide Detection
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Solution Overview
Problem
Current methods lack efficiency and accuracy in identifying stichopus oligopeptide, leading to potential deception of consumers with shoddy products made from other materials.
Innovation Solution
Development of a peptide fragment, monoclonal antibody, colloidal gold test strip, and detection method that utilizes specific peptide fragments and monoclonal antibodies to accurately detect stichopus oligopeptide.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional identification methods are used, then the detection process is simple, but the accuracy and efficiency of identifying stichopus oligopeptide is insufficient
Solution Approach 1:
The patent introduces monoclonal antibodies as intermediary substances that specifically bind to stichopus oligopeptide. These antibodies serve as mediators between the sample and the detection system, enabling accurate identification through antigen-antibody binding reactions. The monoclonal antibodies provide high specificity and sensitivity, resolving the contradiction between detection accuracy and system complexity.
Solution Approach 2:
The patent replaces traditional mechanical or chemical identification methods with immunological detection mechanisms. By using monoclonal antibodies and colloidal gold conjugates, the system substitutes complex mechanical analysis with biological recognition processes, achieving higher measurement precision through molecular-level interactions rather than bulk physical measurement.
2Productivity
If rapid detection methods are developed, then the screening efficiency is improved, but the reliability of identification may be compromised
Solution Approach 1:
The patent employs colloidal gold conjugates that produce visible color changes upon binding to stichopus oligopeptide. The gold-conjugated monoclonal antibodies undergo aggregation and color intensity changes when interacting with the target peptide, providing rapid visual detection. This colorimetric approach enables quick screening while maintaining high reliability through the specificity of antigen-antibody binding.
Solution Approach 2:
The patent utilizes changes in physical and chemical parameters during the detection process, particularly the aggregation state and color properties of colloidal gold particles. When monoclonal antibodies bind to stichopus oligopeptide, the gold particles aggregate and exhibit characteristic color changes, providing a rapid and reliable detection signal that simultaneously achieves high productivity and reliability.
3Measurement precision
If specific monoclonal antibodies are used, then the detection specificity is improved, but the manufacturing complexity increases
Solution Approach 1:
The patent segments the detection system into distinct functional components: monoclonal antibodies for specific recognition, colloidal gold conjugates for signal generation, and test strip elements for implementation. This segmentation allows each component to be optimized independently, with the monoclonal antibodies providing high specificity through their targeted design, while the overall system remains manufacturable through modular assembly.
Solution Approach 2:
The patent uses monoclonal antibodies that can be produced through cell fusion techniques, creating identical copies of the antibody protein. This cloning approach ensures uniformity and reproducibility in the detection system, making the manufacturing process more controllable and scalable while maintaining high detection specificity through the identical nature of the cloned antibody molecules.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves high accuracy and sensitivity, with a detection sensitivity of 20 g/ml, and allows for rapid screening of large quantities of samples, effectively preventing misidentification and ensuring product authenticity.
Implementation Method 1
a monoclonal antibody against KIVPGVPD (SEQ ID NO. 1) or GRDGDQGPV (SEQ ID NO. 3)
Implementation Method 2
a colloidal gold test strip which comprises a sample pad, a gold label pad coated with the above-said monoclonal antibody
Data Source
AI summary
The invention belongs to the field of biotechnology, in particular, relates to peptide fragment, monoclonal antibody, colloidal gold test strip and detection method thereof used for detection of stichopus oligopeptide. Sequences of the peptide fragments are SEQ ID NO: 1 and SEQ ID NO: 3. After identification, the peptides SEQ ID NO: 1 and SEQ ID NO: 3 are newly discovered oligopeptides, which are exclusive peptide fragments of Stichopus oligopeptide.