Structured Illumination Microscopy Using Color CCD and DMD

The patent combines multiple color channel imaging capabilities into a single color CCD camera system. By integrating the imaging of different color channels (red, green, blue) into one camera sensor with appropriate optical filters, the system simultaneously captures full-color information without requiring sequential monochrome imaging, thus resolving the contradiction between color information acquisition and imaging speed.

The patent employs a universal color CCD camera that can capture multiple color channels simultaneously through integrated optical filtering, replacing the need for separate monochrome cameras and complex multi-channel integration systems. This multi-functional approach maintains color imaging capability while significantly simplifying the overall system configuration and reducing the number of components required.

The patent replaces the mechanical point-by-point scanning system with a wide-field optical sectioning approach using structured illumination. Instead of mechanically scanning laser focus through the sample, the system projects structured light patterns and uses computational algorithms to achieve optical sectioning, thereby eliminating mechanical scanning while maintaining spatial resolution and dramatically reducing imaging time.

The patent converts the harmful high-power laser scanning approach into a beneficial low-power wide-field illumination system. By using structured illumination patterns with much lower power requirements and combining them with computational optical sectioning algorithms, the system achieves the same sectioning capability without the phototoxic damage caused by high-power continuous laser exposure.