Color Indicator Test Strip for Oxidative Stress Detection
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Solution Overview
Problem
Current methods for measuring reactive oxygen species in human body fluids, such as lipid peroxides, are complex and require specialized equipment, making it difficult for the general public to detect oxidative stress effectively.
Innovation Solution
A color indicator using 1,3-diethyl-2-thiobarbituric acid (TBA) dissolved in ethanol or aqueous solutions with dimethyl sulfoxide (DMSO) and malonic acid as buffers, enhancing solubility and sensitivity for detecting malondialdehyde in urine or blood, which is an indicator of reactive oxygen species.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional methods (HPLC or TBARS) are used to measure malondialdehyde, then measurement accuracy is maintained, but device complexity and ease of operation deteriorate due to requiring separate apparatuses and expertise
Solution Approach 1:
The patent combines multiple measurement functions into a single integrated test strip that incorporates the color indicator, buffer solution, and reaction substrate. This merging eliminates the need for separate HPLC or TBARS apparatuses while maintaining measurement accuracy through the integrated chemical reaction system.
Solution Approach 2:
The patent introduces a color indicator as an intermediary substance that mediates between malondialdehyde detection and visual measurement. This color indicator system serves as a bridge that translates chemical reactions into observable color changes, enabling accurate measurement without complex instrumentation.
2Measurement precision
If conventional methods (HPLC or TBARS) are used to measure malondialdehyde, then measurement accuracy is maintained, but ease of operation deteriorates due to requiring specialized expertise
Solution Approach 1:
The test strip is designed to perform the measurement function autonomously without requiring specialized expertise. The buffer solution automatically maintains optimal pH conditions, the color indicator self-develops the color reaction, and the substrate provides the reaction environment, enabling any user to perform accurate measurements through simple application.
Solution Approach 2:
The patent optimizes chemical parameters within the test strip, including pH control through buffer solutions and concentration ratios of reagents. These parameter optimizations ensure that the measurement process remains simple and accessible while maintaining high accuracy, as the chemical system self-regulates under optimal conditions.
3Measurement precision
If the color indicator detects low concentrations of malondialdehyde, then detection sensitivity improves, but measurement precision requirements increase
Solution Approach 1:
The patent employs a color indicator that produces distinct color changes in response to low concentrations of malondialdehyde. This color change mechanism amplifies the detection signal, making low-concentration measurements visually distinguishable and reducing the difficulty of detection while maintaining high sensitivity and precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution allows for sensitive detection of low concentrations of malondialdehyde, improving the ability to assess oxidative stress with high discrimination, enabling accurate measurements in urine or blood samples.
Implementation Method 1
a color indicator that detects the amount of reactive oxygen species present in the human body by causing a color change through reaction with malondialdehyde present in the urine and blood
Implementation Method 2
TBA is used in such a manner that it is dissolved in ethanol, an aqueous ethanol solution, or purified water. In this case, the present invention is characterized in that DMSO is added to the ethanol, aqueous ethanol solution, or purified water. The reason for this is to improve the solubility of TBA.
Data Source
AI summary
Provided is, as an attempt to realize a method for measuring oxidative stress in human body fluids, a means for measuring reactive oxygen species containing a color indicator that detects the amount of reactive oxygen species present in the human body by causing a color change through reaction with malondialdehyde present in the urine or blood. The detection means has advantages of detecting malondialdehyde (MDA) present at a low concentration in a sample (urine or blood) and exhibiting excellent discrimination owing to high detection sensitivity.