Compact Monochromatic Light Source for Variable Path Length Measurement

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Solution Overview

Problem

Existing ultraviolet/visible spectrophotometers face challenges in accurately measuring highly concentrated biological samples without dilution, requiring extensive installation space and time-consuming data collection for variable path length measurements.

Innovation Solution

A compact monochromatic light source system using a light emitting diode (LED) coupled with a narrow bandpass filter and a movable optic probe to vary path length, enabling rapid concentration measurements in a compact form factor.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If a UV/visible spectrophotometer system is used for variable path length measurements, then concentration measurement capability is improved, but the system occupies extensive installation space and requires extensive installation effort

Engineering Contradiction:
Improveconcentration measurement capabilityVSAvoidinstallation space
Core Design Contradiction:
Measurement precisionVSArea of stationary object

Solution Approach 1:

The system is divided into separate functional modules: a compact light source unit with LED and bandpass filter, a movable probe assembly with sample chamber, and a detector unit. This segmentation allows each component to be optimized independently and reduces the overall footprint compared to a traditional integrated UV/visible spectrophotometer.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention extracts only the essential components needed for absorption measurements at a specific wavelength, removing unnecessary components of traditional spectrophotometers such as monochromators, gratings, and detectors that scan across broad spectra. This extraction results in a dramatically reduced system size while maintaining concentration measurement capability.

Inventive Principle:
Principle #2Taking out (Extraction)

2Measurement precision

If a UV/visible spectrophotometer system is used for variable path length measurements, then concentration measurement capability is improved, but the measurement time becomes extensive

Engineering Contradiction:
Improveconcentration measurement capabilityVSAvoidmeasurement time
Core Design Contradiction:
Measurement precisionVSLoss of time

Solution Approach 1:

The system uses periodic movement of the probe assembly to vary the optical path length through the sample. The probe moves to predetermined positions corresponding to different path lengths, enabling rapid sequential measurements at multiple path lengths without the time-consuming spectral scanning of traditional instruments.

Inventive Principle:
Principle #19Periodic action

Solution Approach 2:

The invention changes the optical path length parameter by moving the probe assembly to different positions relative to the sample chamber. This allows the system to perform multiple absorbance measurements at different path lengths, enabling concentration determination without dilution while maintaining rapid measurement times.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If a compact light source with narrow bandpass filter is used, then measurement speed is improved, but the light source bandwidth is reduced

Engineering Contradiction:
Improvemeasurement speedVSAvoidlight source bandwidth
Core Design Contradiction:
ProductivityVSLength of moving object

Solution Approach 1:

A narrow bandpass filter is introduced as an intermediary component between the LED light source and the sample. This filter selects a specific wavelength range from the broader LED emission spectrum, providing monochromatic light suitable for absorption measurements while maintaining the compactness and speed advantages of the LED source.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The invention replaces traditional mechanical monochromators with grating or prism-based systems with a simpler LED plus bandpass filter configuration. This substitution eliminates moving mechanical parts for wavelength selection, dramatically reducing measurement time while providing sufficient spectral selectivity for concentration measurements.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The system allows for accurate concentration determination of fluid samples without dilution, reducing measurement time and space requirements, suitable for integration into various processing systems.

Implementation Method 1

a light emitting diode (LED), to generate a probe signal at a first bandwidth

Methodology Applied
Scientific EffectLight emitting diode: Light Emitting Diode

Implementation Method 2

a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth

Methodology Applied
Scientific EffectNarrow bandpass filter: Filter (optical)

Implementation Method 3

Absorption spectroscopy is used to measure composition and/or properties of a material in any phase, gas, liquid, solid

Methodology Applied
Scientific EffectAbsorption spectroscopy: Absorption Spectroscopy

Data Source

PatentUS12596067B2Compact high resolution monochromatic light source for fluid sample concentration measurement
Publication Date: 2026.04.07 REPLIGEN CORP
  • US12596067B2 patent drawing
  • US12596067B2 patent drawing
  • US12596067B2 patent drawing

AI summary

A compact light source for coupling to a movable optic probe may include a light emitting diode (LED), to generate a probe signal at a first bandwidth, an optic coupler, disposed adjacent to the LED, and arranged to couple the probe signal into the movable probe, and a narrow bandpass filter disposed to receive the probe signal at the first bandwidth, and to output the probe signal into the movable probe at a second bandwidth, less than the first bandwidth.