Compact Microscopy Imaging With Marker-Based Scale and Distortion Correction

The patent applies preliminary action by incorporating reference markers with known dimensions directly into the sample holder before imaging. These markers serve as pre-established calibration standards that remain fixed relative to the sample, allowing the system to determine and compensate for position sensitivity without requiring complex real-time adjustments. The reference markers are positioned in advance to provide stable scaling information throughout the imaging process.

The patent uses reference markers as intermediary elements between the sample and the imager. These markers act as mediators that provide known dimensional information, enabling the system to calculate and correct for optical distortions and position variations. The reference markers intermediary allow the system to maintain measurement precision despite the compact configuration and sensitivity to relative positioning.

The patent implements feedback by using reference markers with known dimensions to continuously monitor and provide information about the current optical scale. This feedback mechanism allows the system to detect changes in image scale that occur during focusing operations and to compensate for these changes. The reference markers provide a stable reference that feeds back information about positional variations, enabling correction of scale errors while maintaining focus quality.

The reference markers are pre-positioned in the sample holder before imaging begins, establishing a known dimensional framework in advance. This preliminary placement ensures that regardless of subsequent focusing adjustments, there is always a reference standard available to determine the current scale, allowing the system to maintain both focus quality and scale accuracy simultaneously.

The patent applies universality by designing the sample holder to serve multiple functions: it holds the sample, provides reference markers for calibration, and maintains the sample at the correct position. By integrating the reference markers directly into the sample holder structure, the system eliminates the need for separate calibration devices, thereby reducing overall system complexity while improving calibration accuracy. The sample holder becomes a multi-functional component that simultaneously performs positioning and calibration reference functions.

The patent merges the calibration reference function with the sample holding function by incorporating reference markers directly into the sample holder structure. This combining of functions eliminates the need for separate calibration devices and reduces the number of components required in the system. The reference markers are integrated as part of the sample holder assembly, allowing calibration to be performed using the same structure that holds the sample, thereby improving accuracy without proportionally increasing complexity.

The patent uses reference markers as intermediary elements that provide known dimensional information about the optical system's distortion characteristics. These markers act as mediators between the compact imaging configuration and the optical imperfections, allowing the system to measure and characterize distortion patterns. By having the reference markers in the image field, the system can detect and compensate for lens distortion and illumination variations that are exacerbated by the compact distance between sample and imager.

The reference markers provide feedback information about optical distortions and illumination non-uniformities that occur in the compact imaging configuration. This feedback allows the system to detect distortion patterns and illumination variations caused by the reduced object distance, and to apply corrections to maintain imaging quality. The feedback mechanism enables compensation for the harmful optical effects inherent in compact system design.