Compact Multi-Wavelength Optical Reader for Microplate Assays
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Conventional microplate readers are bulky, slow, and unable to perform simultaneous multi-wavelength readings, which limits their suitability for kinetic assays and temperature control, especially in applications like Endotoxin testing where temperature consistency is crucial.
Innovation Solution
A compact optical reader with a plurality of monochromatic LEDs as light sources and a photodiode array for simultaneous multi-wavelength readings, along with integrated temperature sensors for real-time temperature monitoring and correction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If conventional multi-wavelength microplate readers are used, then multiple wavelengths can be read, but the reading time increases by approximately 100% compared to single wavelength
Solution Approach 1:
The patent divides the microplate reading task into multiple independent wavelength channels, each with its own light source and detector. By segmenting the reading process into parallel wavelength-specific paths, the system can simultaneously acquire data at multiple wavelengths without sequential delays, resolving the contradiction between multi-wavelength capability and reading speed.
Solution Approach 2:
The patent merges multiple wavelength reading paths into a single integrated system that processes all wavelengths simultaneously. By combining the optical paths, control systems, and data processing into one unified multi-wavelength platform, the system achieves parallel reading across all wavelengths, eliminating the time penalty associated with sequential wavelength switching.
2Temperature
If conventional microplate readers are used, then temperature control can be provided, but there is no means to test temperature consistency across the microplate
Solution Approach 1:
The patent implements self-diagnostic temperature verification by using the reader's own optical system to detect temperature-induced refractive index changes in the microplate. The system automatically monitors temperature consistency across the microplate array and generates verification data, enabling the device to self-test and report on its thermal uniformity without requiring external testing equipment.
3Productivity
If kinetic assays are performed with conventional readers, then repeated measurements can be taken, but the speed capabilities are exceeded and kinetic assays cannot be combined within the same microplate
Solution Approach 1:
The patent segments the kinetic measurement process into parallel wavelength-specific detection channels, allowing simultaneous monitoring of multiple kinetic reactions at different wavelengths. This segmentation enables the system to handle high-speed kinetic assays by distributing the measurement load across multiple independent detection paths, preventing speed bottlenecks and enabling combination of multiple kinetic assays within the same microplate.
4Productivity
If bulky stationary multi-wavelength microplate readers are used, then multiple wavelengths can be read simultaneously, but they take up large amounts of space and are not easily movable or stored
Solution Approach 1:
The patent transitions from a traditional horizontal benchtop configuration to a vertical or space-efficient compact arrangement by reorienting the optical paths and component layout. The system uses vertical stacking of optical components, folded light paths, and compact mechanical designs that reduce the horizontal footprint while maintaining simultaneous multi-wavelength reading capability, allowing the device to fit on smaller worktops and be more easily moved or stored.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid, simultaneous multi-wavelength readings of microplate samples with minimal increase in time, significantly improving throughput and allowing for precise temperature control and correction, enhancing the accuracy of optical data in temperature-sensitive assays.
Implementation Method 1
an illuminating array including a plurality of light sources of predetermined differing-wavelength outputs
Implementation Method 2
a detector operatively configured and located to detect light from the predetermined differing-wavelength outputs from the illuminating array
Data Source
AI summary
An optical reader having an array of differing-color light sources and a controller for controlling the light sources and acquisition of optical data. The light sources are arranged, and the controller is configured, to allow rapid acquisition of optical data regarding individual sample wells of a cluster of such wells. In some embodiments, multiple ones of the differing-color light sources are illuminated simultaneously for acquiring optical data on a corresponding number of sample wells. Depending on the configuration of the array and number of differing-color light sources illuminated simultaneously, the optical reader can acquire optical data for several wavelengths in a fraction of the time of conventional optical readers. Other embodiments include one or more non-contact temperature sensors for acquiring temperature data substantially simultaneously with the optical data. The temperature data can be used, for example, to adjust the optical data or warn a user of out-of-specification temperature conditions.