Complex Polysaccharide Regulating Tumor Microenvironment
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Solution Overview
Problem
Current tumor therapies focus primarily on tumor cells, neglecting the importance of the tumor microenvironment, leading to challenges in preventing recurrence and metastasis, while polysaccharides have shown promise in anti-tumor activity with minimal side effects, but their application in regulating tumor microenvironments is not fully explored.
Innovation Solution
A Lucid Ganoderma, Lycium barbarum, and Polygonatum sibiricum complex polysaccharide is developed, with specific mass proportion ratios of each component, extracted using a method involving boiling water, dialysis, trichloroacetic acid, and ethanol precipitation, to create a formulation suitable for industrial production and use in health-care foods for regulating tumor microenvironments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If current tumor therapy focuses on tumor cells, then tumor cell targeting is improved, but tumor microenvironment regulation deteriorates
Solution Approach 1:
The patent combines three different polysaccharides (lucid ganoderma polysaccharide, lycium barbarum polysaccharide, and polygonatum sibiricum polysaccharide) into a complex formulation. This merging of multiple bioactive components addresses the limitation of single-target therapy by simultaneously targeting tumor cells and regulating the tumor microenvironment, including angiogenesis inhibition and immune modulation.
Solution Approach 2:
The invention uses a composite polysaccharide material comprising multiple types of polysaccharides in specific ratios. This composite approach leverages the synergistic effects of different polysaccharides to achieve both tumor cell targeting and microenvironment regulation, resolving the contradiction between precise targeting and comprehensive regulation.
2Manufacturing precision
If polysaccharide complex is extracted using multiple processing steps, then extraction purity is improved, but processing complexity deteriorates
Solution Approach 1:
The extraction process is divided into distinct sequential steps: extraction, dialysis, precipitation, and drying. Each step isolates and purifies the polysaccharide complex through a specific mechanism, achieving high purity while maintaining manageable process complexity through systematic segmentation of the overall extraction procedure.
Solution Approach 2:
The patent uses dialysis and precipitation as intermediary steps to separate the polysaccharide complex from other cellular components. These intermediary processes act as purification barriers that progressively remove impurities without requiring overly complex equipment, balancing purity achievement with process simplicity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The complex polysaccharide effectively inhibits tumor angiogenesis, immunosuppression, and tumor growth, demonstrating good anti-tumor activity and safety for oral administration, and can be formulated into various pharmaceutical forms like capsules or oral liquids.
Implementation Method 1
polysaccharide has a significant anti-tumor activity and has a rather small toxic side effect on human body
Implementation Method 2
having effects for regulating tumor microenvironment
Implementation Method 3
extracted using a method involving boiling water, dialysis, trichloroacetic acid, and ethanol precipitation
Implementation Method 4
trichloroacetic acid, and ethanol precipitation
Data Source
AI summary
The present invention discloses a Lucid Ganoderma, Lycium barbarum and Polygonatum sibiricum complex polysaccharide having efficacy for regulating tumor microenvironment, whose active components are complexed by Lucid Ganoderma polysaccharide, Lycium barbarum polysaccharide and Polygonatum sibiricum polysaccharide, the mass proportion ratio of each component is Lucid Ganoderma polysaccharide 10-250, Lycium barbarum polysaccharide 1-50, Polygonatum sibiricum polysaccharide 2-10. The Lucid Ganoderma, Lycium barbarum and Polygonatum sibiricum complex polysaccharide is high in purity and activity, has an efficacy for regulating tumor microenvironment. The present invention also discloses the preparation method of aforesaid Lucid Ganoderma, Lycium barbarum and Polygonatum sibiricum complex polysaccharide, said method is simple, stable and efficient in the process, suitable for industrial production and economic. In addition, the present invention further discloses the use of aforesaid Lucid Ganoderma, Lycium barbarum and Polygonatum sibiricum polysaccharide in the preparation of health-care food having efficacy for regulating tumor microenvironment.