Component Analysis Boundary Determination via Waveform Inversion
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Solution Overview
Problem
In component analysis systems using continuous sample introduction, such as capillary electrophoresis, it is challenging to distinctly determine the boundary between peaks, especially when peaks are merged, leading to difficulties in identifying and quantifying components.
Innovation Solution
A method that involves continuously introducing a sample solution into a flow path, optically measuring it over time, and analyzing the data to acquire a base waveform, differentiate it, and determine measurement value boundaries between components, even in cases where traditional peak boundaries are indistinct.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional peak boundary determination methods are used in differentiated waveforms, then simple analysis is possible, but boundary determination becomes inaccurate when peaks are merged
Solution Approach 1:
Instead of determining boundaries at the bottom of peaks in the differentiated waveform, the invention inverts the approach by determining boundaries at the peak tops. This inversion allows accurate boundary identification even when peaks are merged, as the peak tops remain distinguishable features in the differentiated waveform
Solution Approach 2:
The invention transitions from analyzing the differentiated waveform alone to considering both the base waveform and differentiated waveform together. By examining the relationship between these two dimensions (original signal and its derivative), the method achieves more reliable boundary determination than either waveform could provide independently
2Ease of operation
If valley portions are used as boundaries between peaks, then boundary identification is straightforward, but boundaries become indistinct when peaks are merged
Solution Approach 1:
The invention inverts the traditional boundary identification approach by using peak tops instead of valley bottoms. This inversion maintains ease of operation through automated peak detection while significantly improving accuracy in merged peak scenarios where valleys are indistinct or absent
Solution Approach 2:
The differentiated waveform serves as an intermediary that transforms the original base waveform into a form where peak boundaries are more clearly defined. By detecting peaks in this intermediate differentiated representation, the method achieves both ease of automated operation and high boundary determination accuracy
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables definitive boundary determination between components, allowing for accurate identification and quantification, even in cases where peaks are merged or boundaries are not distinct, thereby improving the analysis precision.
Implementation Method 1
optically measured over time at a measurement position on the flow path to obtain optical measurement values
Implementation Method 2
a measurement value differentiated waveform is acquired, which is a waveform obtained by differentiating the base waveform along an axis of the optical measurement values orthogonal to the time axis
Data Source
AI summary
The present disclosure provides component analysis methods including a measurement process and an analysis process.