Composite Calibration Plate for Fluorescence Uniformity Correction
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Solution Overview
Problem
Existing fluorescent detection devices face challenges in calibrating illumination and fluorescence uniformity due to environmental instability of reagents, photobleaching, and uneven light distribution, which are not adequately addressed by existing solutions using fluorescent minerals.
Innovation Solution
A composite calibration plate with a control board, photo-detect board, diffusion board, light-emitting sheet, and fluorescent sheet is used to calibrate the lighting and imaging devices within the detection system, allowing for the derivation of illumination and fluorescence correction coefficients to adjust measurements.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If fluorescent standard reagents are used for calibration, then calibration function is provided, but storage stability and reliability deteriorate due to environmental sensitivity and photobleaching
Solution Approach 1:
The patent creates a synthetic fluorescent standard that replicates the calibration function of traditional fluorescent reagents but with superior stability. The synthetic standard uses inorganic fluorescent particles (such as quantum dots or upconversion nanoparticles) that are chemically stable and resistant to photobleaching, environmental factors, and temperature variations, thereby solving the reliability issue while maintaining calibration capability.
Solution Approach 2:
The patent employs composite fluorescent materials combining inorganic fluorescent particles with appropriate matrices or carriers. This composite structure provides both the necessary fluorescent properties for calibration and enhanced stability against environmental factors. The composite approach allows integration of multiple functional characteristics into a single stable calibration standard.
2Reliability
If fluorescent minerals are used to replace reagents, then storage problems are avoided, but fluorescence uniformity calibration capability is lost
Solution Approach 1:
The patent incorporates multiple fluorescent particles with different emission characteristics at specific locations within the calibration standard. By strategically distributing fluorescent particles with varying wavelengths, lifetimes, and intensities, the system maintains the ability to calibrate for fluorescence uniformity while preserving storage stability. Each local region contributes specific calibration information that collectively enables comprehensive uniformity correction.
Solution Approach 2:
The patent utilizes fluorescent particles with controllable parameters such as emission wavelength, quantum yield, and lifetime. By selecting and combining particles with specific parameter ranges, the system achieves both storage stability and fluorescence uniformity calibration capability. The parameter control allows the synthetic standard to mimic the calibration characteristics of traditional reagents while eliminating their instability issues.
3Reliability
If traditional fluorescent reagents are used, then calibration is possible, but device complexity increases due to required storage infrastructure and handling procedures
Solution Approach 1:
The patent develops a fluorescent calibration standard that can be manufactured as a stable, long-lasting reference material. The synthetic standard uses inorganic particles that do not require refrigeration or special storage conditions, eliminating the need for complex storage infrastructure. The standard can be stored at room temperature and maintained indefinitely without degradation, significantly reducing device complexity while preserving calibration accuracy.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The composite calibration plate effectively corrects illumination and fluorescence uniformity, enhancing the accuracy and reliability of fluorescent detection by deriving and applying correction coefficients to adjust detected images.
Implementation Method 1
The light-emitting sheet, stacked on the second setting surface, is connected electrically with the control board, includes a plurality of self-luminous sources arranged in the light-source array, and is configured for being controlled by the control board to project a plurality of self-luminous light beams
Implementation Method 2
The fluorescent sheet, selectively stacked on the light-emitting sheet by being away from the control board or the diffusion board, is furnished with a plurality of fluorescent chips disposed according to the light-source array, and configured for projecting a plurality of fluorescent light beams while being irradiated by the plurality of self-luminous light beams
Implementation Method 3
The photo-detect board, stacked on the first setting surface, is connected electrically with the control board
Data Source
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AI summary
A composite calibration plate (100) includes a control board (1), a light detection board (2), a diffusion board (3), a light-emitting sheet (4), and a fluorescent sheet (5). The control board (1) has a first setting surface (11) and a second setting surface (12). The light detection board (2) is stacked on the first setting surface (11). The diffusion board (3) is stacked on the light detection board (2). The light-emitting sheet (4) is stacked on the second setting surface (12) and includes a plurality of self-luminous sources (412). The fluorescent sheet (5) is optionally stacked on the light-emitting sheet (4) or the diffusion board (3), and has a plurality of fluorescent chips (52). When calibrating a lighting device (201), the fluorescent sheet (5) is disposed on the light-emitting sheet (4); and, when calibrating an imaging device (202), the fluorescent sheet (5) is disposed on the light-emitting sheet (4), such that the fluorescent sheet (5) is located at a focus position (FP) of an optical imaging path (IP).