Composite Collagen Processing for Mechanical Strength and Enzyme Resistance
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Solution Overview
Problem
Collagen's weak mechanical properties and susceptibility to enzymatic degradation pose challenges in biomaterial applications, limiting its effectiveness in various products.
Innovation Solution
A process is developed to produce soluble and insoluble collagen products from mammalian dermis tissue, involving enzymatic treatment, lipid removal, homogenization, and separation techniques, followed by chemical or physical crosslinking to enhance mechanical stability and durability.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If collagen is used in biomaterial applications, then biocompatibility and biodegradability are improved, but mechanical strength and resistance to enzymatic degradation deteriorate
Solution Approach 1:
The patent applies composite materials by combining collagen with other materials (such as synthetic polymers, natural polymers, or inorganic materials) to create composite collagen products. This composite structure allows the material to retain the biocompatibility and biodegradability of collagen while gaining enhanced mechanical strength and enzymatic degradation resistance from the additional materials.
2Duration of action of stationary object
If collagen is used in biomaterial applications, then biodegradability is improved, but resistance to enzymatic degradation deteriorates
Solution Approach 1:
By creating composite collagen products with materials that have different degradation rates and enzymatic resistance properties, the invention achieves a balanced performance where the product maintains controlled biodegradability while exhibiting improved resistance to enzymatic degradation compared to pure collagen.
3Productivity
If dermis tissue is minced into smaller pieces, then processing speed is improved, but surface area for extraction is increased
Solution Approach 1:
The patent applies segmentation by mincing dermis tissue into smaller pieces, which divides the large tissue mass into numerous small fragments. This segmentation increases the total surface area available for enzymatic extraction and chemical treatment, thereby improving the efficiency and speed of collagen extraction while maintaining controlled processing conditions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The process yields collagen products with tailored mechanical and biological properties, suitable for applications requiring strength and resistance to enzymatic degradation, such as bone and cartilage tissue engineering.
Implementation Method 1
The minced dermis tissue may be washed in a lipid removal solution that is an amylase solution comprising an effective amount of amylase to remove the lipids and soften the tissue for extraction
Implementation Method 2
An extracted collagen solution may undergo centrifugation to physically separate the soluble from the insoluble collagen
Data Source
AI summary
A process for the production of a soluble collagen product and an insoluble collagen product from mammalian dermis tissue is described. The tissue may initially be processed to remove the epidermis and adipose tissue and then minced into small pieces. The process includes washing the minced dermis in an enzymatic solution, such as an amylase solution followed by homogenizing the amylased tissue. The soluble and insoluble collagen is then extracted from the homogenized tissue and subsequently separated into distinct soluble and insoluble fractions. The process produces collagen with high Dalton values that can subsequently be combined to produce a mixed composite collagen product. Also, the extracted collagen may be used as an integral composite collagen before separation.