Composite Nano-agent for Combating Drug-fast Bacteria

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Solution Overview

Problem

The challenge is to develop an effective composite nano-agent for combating drug-fast bacteria, as existing methods face difficulties in efficiently preparing silver nanoparticles due to limited penetration of ultraviolet light, which affects the bactericidal efficacy of silver nanoparticles in deeper layers.

Innovation Solution

A composite nano-agent comprising silver nanoparticles, silver ions, polyvinyl alcohol (PVA), polyethylene glycol (PEG), tetrabutylammonium hydroxide (TBAH), and liposomes, where silver ions are reduced in a reduction equipment with enhanced ultraviolet light irradiation, and liposomes are formed through ultrasonic treatment to improve stability and distribution, with PEG added to stabilize the mixture.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If ultraviolet light is used to reduce silver ions in a mixture, then silver nanoparticles can be prepared, but the penetration power of ultraviolet light is limited and it is difficult to effectively irradiate deeper layers

Engineering Contradiction:

Improvepreparation efficiency of silver nanoparticlesVSAvoidpenetration power of ultraviolet light

Core Design Contradiction:

ProductivityVSIllumination intensity

Solution Approach 1:

The reduction tank is divided into multiple layers by partition plates, creating segmented reaction zones. This segmentation allows ultraviolet light to irradiate each layer effectively while maintaining overall high preparation efficiency, as each segmented layer receives adequate light penetration for silver ion reduction.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The invention transitions from a single-layer reduction approach to a multi-layer segmented structure. By adding the vertical dimension of multiple irradiatable layers, the system overcomes the limited penetration depth of ultraviolet light while maintaining high productivity through simultaneous multi-zone reduction reactions.

Inventive Principle:

Principle #17Another dimension (Dimensionality change)

2Reliability

If silver nanoparticles are prepared using conventional methods, then bactericidal activity is achieved, but biocompatibility and stability are insufficient

Engineering Contradiction:

Improvebactericidal activityVSAvoidbiocompatibility and stability

Core Design Contradiction:

ReliabilityVSStability of the object's composition

Solution Approach 1:

The invention creates a composite nano-agent system combining silver nanoparticles, PVA, PEG, and liposomes. This composite structure maintains the bactericidal activity of silver nanoparticles while the PVA and PEG coatings enhance biocompatibility and stability, and liposomes provide additional protective and delivery functions.

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

PVA and PEG act as intermediary substances between the silver nanoparticles and the biological environment. These intermediaries coat the silver nanoparticles, reducing their direct interaction with biological systems while maintaining their bactericidal function, thereby improving biocompatibility and stability.

Inventive Principle:

Principle #24Intermediary (Mediator)

3Productivity

If TBAH is added to hydrolyze silver salt, then silver ions are generated efficiently, but the ratio control between silver salt and TBAH must be precise

Engineering Contradiction:

Improvegeneration efficiency of silver ionsVSAvoidratio control of silver salt to TBAH

Core Design Contradiction:

ProductivityVSManufacturing precision

Solution Approach 1:

The invention optimizes and specifies the molar ratio parameter of silver salt to TBAH within the range of 1:1 to 1:3. By establishing this parameter range, the system achieves efficient silver ion generation while simplifying the precision requirements for ratio control, allowing flexible preparation within the optimal range.

Inventive Principle:

Principle #35Parameter changes

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method enables efficient preparation and stabilization of silver nanoparticles, enhancing their bactericidal properties and biocompatibility, thereby effectively combating drug-fast bacteria with improved sterilization effects and minimal inhibitory concentration (MIC).

Implementation Method 1

adding TBAH to hydrolyze the silver salt

Methodology Applied

Scientific EffectHydrolysis: Hydrolysis

Implementation Method 2

reducing the silver ions into the silver nanoparticles in reduction equipment

Methodology Applied

Scientific EffectReduction: Reduction

Implementation Method 3

carrying out ultrasonic treatment to form the liposomes

Methodology Applied

Scientific EffectUltrasonic vibration: Ultrasonic Vibration

Implementation Method 4

adding PEG to stabilize the mixture

Methodology Applied

Scientific EffectSteric stabilization:

Data Source

PatentUS20240099978A1Composite Nano-agent for Combating Drug-fast Bacteria and Preparation Method Thereof

Publication Date: 2024.03.28 GUANGDONG MEDICAL UNIV

AI summary

Disclosed is a composite nano-agent for combating drug-fast bacteria and a preparation method thereof. The solution comprises several steps of operation that a silver salt is reduced into silver nanoparticles; then, phosphatidylcholine (PC) and cholesterol are mixed and added into water, and ultrasonic treatment is carried out to form liposomes; the silver nanoparticles and the liposomes are then mixed and an ultrasonic processor is used to uniformly mix a mixture by adding polyethylene glycol (PEG); an anti-bacterial experiment is carried out on the prepared reagent to determine a sterilization effect and a minimal inhibitory concentration (MIC) thereof.