Conditioned Decellularized Matrix for Tissue Regeneration
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Solution Overview
Problem
Current tissue graft constructs, such as regenerative scaffolds, have limitations in supporting the replication, differentiation, maturation, and spatial organization of various cell types, particularly due to environmental and physiological factors, necessitating improved compositions for tissue repair, replacement, and remodeling.
Innovation Solution
A composition comprising a decellularized matrix conditioned by cells cultured in vitro, which can include naturally occurring extracellular matrix components or their degradation products, with cells expressing biologically active molecules and exposed to stressors like hypoxia, mechanical loading, or electrical currents to enhance restorative and remodeling properties.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If naturally occurring ECM is used as a scaffold matrix, then the scaffold provides structural support and biological activity, but it lacks enhanced restorative properties for supporting cell replication, differentiation, and spatial organization
Solution Approach 1:
The patent applies preliminary action by culturing cells on the ECM matrix before implantation to pre-condition the scaffold with growth factors and cellular components. This pre-conditioning enhances the matrix's restorative properties and its ability to support cell replication, differentiation, and spatial organization when implanted, without requiring complex modifications during or after implantation.
2Reliability
If cells are cultured on the matrix in vitro to condition the matrix, then the matrix gains enhanced restorative properties, but the process requires additional time and resources
Solution Approach 1:
The patent applies parameter changes by optimizing cell culture conditions including exposure to stressors (hypoxia, mechanical loading, electrical currents) and controlling environmental factors (pH, ionic concentrations, oxygen levels). These parameter modifications enhance the conditioning process efficiency, enabling the matrix to acquire improved restorative properties within a practical timeframe suitable for clinical application.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The conditioned matrix demonstrates enhanced restorative, remodeling, and repair properties when implanted, closely mimicking natural tissue regeneration by supporting cellular replication, differentiation, and spatial organization, thereby improving tissue repair and replacement outcomes.
Implementation Method 1
The matrix is conditioned by cells cultured on the matrix in vitro
Implementation Method 2
cells expressing biologically active molecules
Implementation Method 3
exposed to stressors like hypoxia
Implementation Method 4
mechanical loading
Implementation Method 5
electrical currents
Data Source
AI summary
The invention provides a composition conditioned for the remodeling, restoration, repair, or replacement of tissue within a host. The composition is conditioned by culturing cells on the matrix and/or by exposing the cultured cells or matrix to one or more stressors.