Conditioned Media Co-Culture for Balanced Secreted Factor Profiles

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Solution Overview

Problem

Existing methods for producing conditioned media and secreted factors lack standardization, leading to inconsistent profiles and undesired side effects when used therapeutically, and fail to replicate physiologically balanced levels for scalable applications.

Innovation Solution

A method for producing Consortia Media and Consortia Factors through co-culturing cells from different sources, optimizing cell-cell interactions to achieve chemically and physiologically balanced factor profiles, allowing for scalable and consistent production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If complex culture media are used to maintain original phenotype, then cell phenotype maintenance is improved, but manufacturing precision and consistency of secreted factors deteriorate

Engineering Contradiction:

Improvecell phenotype maintenanceVSAvoidconsistency of secreted factors

Core Design Contradiction:

ReliabilityVSManufacturing precision

Solution Approach 1:

The patent changes the parameters of culture media by defining specific compositions with controlled concentrations of growth factors, hormones, and nutrients. This standardization allows consistent production of secreted factors while maintaining cell phenotype through optimized cultural conditions rather than complex undefined media

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent segments the culture process into controlled stages with specific media formulations for different cell types and differentiation states. This enables precise control over secreted factor profiles while maintaining phenotype consistency across production batches

Inventive Principle:

Principle #1Segmentation

2Productivity

If cells are cultured in vitro to produce secreted factors, then production scalability is improved, but physiological balance of factor profiles deteriorates

Engineering Contradiction:

Improveproduction scalabilityVSAvoidphysiological balance of factor profiles

Core Design Contradiction:

ProductivityVSStability of the object's composition

Solution Approach 1:

The patent uses conditioned media from primary tissue cultures as an intermediary to transfer physiological balance to subsequent cell culture systems. This mediator contains balanced profiles of growth factors and cytokines that guide scalable cell cultures to produce physiologically relevant factor profiles

Inventive Principle:

Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent performs preliminary conditioning of culture media using primary cell cultures before using them for large-scale production. This preliminary action establishes the physiological balance in the media that is then maintained during scalable production phases

Inventive Principle:

Principle #10Preliminary action

3Manufacturing precision

If standardized production methods are implemented, then manufacturing precision is improved, but adaptability to different cell types deteriorates

Engineering Contradiction:

Improvestandardization of productionVSAvoidadaptability to different cell types

Core Design Contradiction:

Manufacturing precisionVSAdaptability or versatility

Solution Approach 1:

The patent creates a universal platform methodology that can be applied to different cell types through modular media formulations. The standardized production framework includes adaptable protocols that maintain precision while accommodating various cell types through parameter adjustments rather than requiring entirely separate standardized methods for each cell type

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentUS12569537B2System and method for the production, formulation and use of conditioned media, cultured cells and the factors included therein

Publication Date: 2026.03.10 RINATI SKIN LLC

AI summary

A technology regarding the production, formulation and use of conditioned media and the factors included therein is disclosed. The conditioned media may be inoculated with animal cells, plant cells and any combination thereof. The inoculations may occur simultaneous or at different times. Cells retrieved from different areas of the animal and/or the plant may also be cultured together to form conditioned media and associated growth factors.